Elias Júlio César Ferreira, Gonçalves-Vidigal Maria Celeste, Vaz Bisneta Mariana, Valentini Giseli, Vidigal Filho Pedro Soares, Gilio Thiago Alexandre Santana, Moda-Cirino Vânia, Song Qijian
Departamento de Agronomia, Universidade Estadual de Maringá-UEM, Av. Colombo 5790, Maringá 87020-900, PR, Brazil.
Soybean Genomics and Improvement Laboratory, US Department of Agriculture, Agricultural Research Service (USDA-ARS), Beltsville, MD 20705, USA.
Plants (Basel). 2021 Jul 30;10(8):1568. doi: 10.3390/plants10081568.
One of the significant challenges of common bean breeding is developing cultivars with high yields under drought conditions. The present study attempted to map quantitative trait loci (QTLs) and identify molecular markers that are linked to drought tolerance in the common bean. We evaluated 160 recombinant inbred lines (RILs), derived from the cross between the carioca cultivars IAPAR 81 (drought tolerant) and LP97-28 (susceptible to drought). In 2014 and 2015, two experiments were conducted (DS-drought stress, and NS-no drought stress). In the DS experiment, water suppression was performed at the flowering stages R5 to R6. The results of our experiments showed that drought conditions play an essential role in reducing most of the traits that were evaluated. RILs under drought conditions reduced the grain yield by 62.03% and 24% in 2014 and 2015, respectively. We identified 15 quantitative trait loci distributed on the chromosomes Pv01, Pv02, Pv03, Pv07, Pv08, Pv09, Pv10, and Pv11, related to grain yield, seed yield per day, 100-seed weight, number of pods per plant, plant height, number of days for flowering, and number of days to maturity. The characteristics of seed yield per day, 100-seed weight, and number of days to maturity showed that QTLs colocalized on Pv07. Identifying QTLs that are linked to drought tolerance in the RIL population IAPAR 81 × LP97-28 is of particular importance for common bean breeding programs seeking to improve carioca beans that are cultivated in regions with drought conditions, such as Brazil.
普通菜豆育种的重大挑战之一是培育出在干旱条件下高产的品种。本研究试图绘制数量性状基因座（QTL）图谱，并鉴定与普通菜豆耐旱性相关的分子标记。我们评估了160个重组自交系（RIL），这些自交系源自carioca品种IAPAR 81（耐旱）和LP97 - 28（干旱敏感）的杂交。在2014年和2015年进行了两项试验（DS - 干旱胁迫，NS - 无干旱胁迫）。在DS试验中，在开花期R5至R6进行水分抑制。我们的试验结果表明，干旱条件对降低所评估的大多数性状起着至关重要的作用。干旱条件下的RIL在2014年和2015年分别使籽粒产量降低了62.03%和24%。我们鉴定出15个数量性状基因座，分布在Pv01、Pv02、Pv03、Pv07、Pv08、Pv09、Pv10和Pv11染色体上，与籽粒产量、每日种子产量、百粒重、单株荚数、株高、开花天数和成熟天数有关。每日种子产量、百粒重和成熟天数的特征表明，QTL共定位在Pv07上。对于旨在改良在巴西等干旱地区种植的carioca菜豆的普通菜豆育种计划而言，在RIL群体IAPAR 81×LP97 - 28中鉴定与耐旱性相关的QTL尤为重要。
