Lindsay Donna L, Jha Ambuj B, Arganosa Gene, Glahn Raymond, Warkentin Thomas D
Crop Development Centre, Department of Plant Sciences, University of Saskatchewan, Saskatoon, SK S7N 5A8, Canada.
Robert W Holley Ctr Ag & Health, USDA-ARS, Cornell University, Ithaca, NY 14853-2901, USA.
Plants (Basel). 2021 Aug 2;10(8):1589. doi: 10.3390/plants10081589.
Field pea is a pulse that delivers high protein content, slowly digestible starch and fiber, and many vitamins and minerals, including iron. Naturally occurring plant phytic acid molecules bind iron, lowering its availability for absorption during digestion. Two low phytic acid () pea lines, 1-2347-144 and 1-150-81, developed by our group had 15% lower yield and 6% lower seed weight relative to their progenitor cultivar. Subsequently, we crossed the two lines and two cultivars, and derived 19 promising pea breeding lines; here we document their agronomic performance based on 10 replicated field trials in Saskatchewan. Seventeen of these lines yielded greater than 95% of the check mean (associated cultivars) and 16 were above 98% of the check mean for 1000 seed weight. The 19 lines showed 27 to 55% lower phytic acid concentration than the check mean. Iron concentrations were similar in all the lines and cultivars, yet the Caco-2 human cell culture assay revealed 14 of the 19 lines had 11 to 55% greater iron bioavailability than check means. Thus, a single round of plant breeding has allowed for closing the gap in performance of low phytic acid pea.
豌豆是一种富含高蛋白、难消化淀粉和纤维以及多种维生素和矿物质（包括铁）的豆类。天然存在的植物植酸分子会与铁结合，从而降低其在消化过程中的吸收利用率。我们团队培育的两个低植酸（）豌豆品系1-2347-144和1-150-81，相对于它们的原始品种，产量降低了15%，种子重量降低了6%。随后，我们将这两个品系与两个品种进行杂交，获得了19个有前景的豌豆育种品系；在此，我们基于在萨斯喀彻温省进行的10次重复田间试验记录了它们的农艺性能。这些品系中有17个的产量超过对照平均值（相关品种）的95%，16个的千粒重超过对照平均值的98%。这19个品系的植酸浓度比对照平均值低27%至55%。所有品系和品种中的铁浓度相似，但Caco-2人细胞培养试验表明，这19个品系中有14个的铁生物利用率比对照平均值高11%至55%。因此，一轮植物育种就缩小了低植酸豌豆在性能上的差距。
