Liu Zhuoxing, Zhang Dasheng, Zhang Weiwei, Xiong Lei, Liu Qingqing, Liu Fengluan, Li Hanchun, An Xiangjie, Cui Lijie, Tian Daike
Shanghai Key Laboratory of Plant Functional Genomics and Resources, Shanghai Chenshan Plant Science Research Center of Chinese Academy of Sciences, Shanghai Chenshan Botanical Garden, Shanghai 201602, China.
Development Center of Plant Germplam Resources, College of Life Science, Shanghai Normal University, Shanghai 200234, China.
Plants (Basel). 2021 Aug 9;10(8):1629. doi: 10.3390/plants10081629.
The lotus ( Adans.) is an important aquatic plant with ornamental, medicinal and edible values and cultural connotations. It has single-, semi-double-, double- and thousand-petalled types of flower shape and is an ideal material for developmental research of flower doubling. The lotus is a basal eudicot species without a morphological difference between the sepals and petals and occupies a critical phylogenetic position in flowering plants. In order to investigate the genetic relationship between the sepals and petals in the lotus, the class genes which affect sepal formation were focused on and analyzed. Here, () and its homologous genes () and () of the class gene family were isolated from the flower buds of the Asian lotus ( Gaertn.). The protein structure, subcellular localization and expression patterns of these three genes were investigated. All three genes were verified to locate in the nucleus and had typical MADS-box characteristics. and were specifically expressed in the sepals, while was highly expressed in the petals, suggesting that different developmental mechanisms exist in the formation of the sepals and petals in the lotus. The significant functional differences between NnSEP1, NnMADS6-like and NnAGL9 were also confirmed by a yeast two-hybrid assay. These results expand our knowledge on the class gene family in sepal formation and will benefit fundamental research on the development of floral organs in
莲（睡莲科莲属）是一种重要的水生植物，具有观赏、药用、食用价值及文化内涵。它有单瓣、半重瓣、重瓣和千瓣等花型，是花发育加倍研究的理想材料。莲是基部真双子叶植物，萼片和花瓣在形态上无差异，在开花植物中占据关键的系统发育位置。为了研究莲中萼片和花瓣之间的遗传关系，重点关注并分析了影响萼片形成的 类基因。在此，从亚洲莲（莲）的花芽中分离出了 类基因家族的（）及其同源基因（）和（）。对这三个基因的蛋白质结构、亚细胞定位和表达模式进行了研究。证实这三个基因均定位于细胞核且具有典型的MADS盒特征。和 在萼片中特异性表达，而 在花瓣中高表达，表明莲中萼片和花瓣的形成存在不同的发育机制。酵母双杂交试验也证实了NnSEP1、NnMADS6-like和NnAGL9之间存在显著的功能差异。这些结果拓展了我们对 类基因家族在萼片形成方面的认识，并将有助于花器官发育的基础研究 。
