Vitale Ermenegilda, Vitale Luca, Costanzo Giulia, Velikova Violeta, Tsonev Tsonko, Simoniello Palma, De Micco Veronica, Arena Carmen
Department of Biology, University of Naples Federico II, Via Cinthia, 80126 Napoli, Italy.
National Research Council, Department of Biology, Agriculture and Food Sciences, Institute for Agricultural and Forestry Systems in the Mediterranean, P. le E. Fermi 1, 80055 Portici, Italy.
Plants (Basel). 2021 Aug 23;10(8):1752. doi: 10.3390/plants10081752.
This study evaluated if specific light quality (LQ) regimes (white fluorescent, FL; full-spectrum, FS; red-blue, RB) during plant growth modified morphological and photosynthetic traits of L. 'Microtom' plants irradiated at the dry seed stage with 25 Gy Ca ions (IR). The irradiation reduced plant size while it increased leaf dry matter content (LDMC) and relative water content (RWC) compared to the control. FS and RB light regimes determined a decrease of plant height and a rise of RWC compared to FL plants. The irradiation under FS and RB regimes favoured the development of dwarf plants and improved the leaf water status. Under the FL regime, irradiated plants showed reduced photosynthesis and stomatal conductance. The opposite behavior was observed in RB irradiated plants in which gas exchanges were significantly stimulated. RB regime enhanced Rubisco expression in irradiated plants also inducing anatomical and functional adjustments (i.e., increase of leaf thickness and incidence of intercellular spaces). Finally, Ca ions did not prevent fruit ripening and the achievement of the 'seed-to seed' cycle, irrespective of the LQ regime. Overall, the present study evidenced that RB light regime was the most effective in optimising growth and photosynthetic efficiency of 'Microtom' irradiated plants. These outcomes may help to develop proper cultivation protocols for the growth of dwarf tomato in Controlled Ecological Life Support Systems (CELSS).
本研究评估了在植物生长期间特定光质（LQ）模式（白色荧光灯，FL；全光谱，FS；红蓝光，RB）是否会改变在干种子阶段用25 Gy钙离子（IR）辐照的‘Microtom’番茄植株的形态和光合特性。与对照相比，辐照降低了植株大小，同时增加了叶片干物质含量（LDMC）和相对含水量（RWC）。与FL植株相比，FS和RB光质模式使植株高度降低，RWC升高。FS和RB模式下的辐照有利于矮化植株的发育，并改善了叶片水分状况。在FL模式下，辐照植株的光合作用和气孔导度降低。在RB辐照植株中观察到相反的情况，其气体交换受到显著刺激。RB模式增强了辐照植株中Rubisco的表达，还诱导了解剖学和功能上的调整（即叶片厚度增加和细胞间隙发生率增加）。最后，无论LQ模式如何，钙离子都不会阻止果实成熟和‘从种子到种子’周期的完成。总体而言，本研究证明RB光质模式在优化辐照的‘Microtom’植株的生长和光合效率方面最有效。这些结果可能有助于为受控生态生命支持系统（CELSS）中矮化番茄的生长制定适当的栽培方案。
