Balestrin Lucélia Albarello, Kreutz Tainá, Fachel Flávia Nathiely Silveira, Bidone Juliana, Gelsleichter Nicolly Espindola, Koester Letícia Scherer, Bassani Valquiria Linck, Braganhol Elizandra, Dora Cristiana Lima, Teixeira Helder Ferreira
Programa de Pós-Graduação em Ciências Farmacêuticas, Universidade Federal do Rio Grande do Sul, Porto Alegre 90610-000, Brazil.
Curso de Farmácia, Centro de Ciências Químicas, Farmacêuticas e de Alimentos, Universidade Federal de Pelotas, Pelotas 96010-900, Brazil.
Pharmaceutics. 2021 Aug 12;13(8):1241. doi: 10.3390/pharmaceutics13081241.
(Lam.) DC Asteraceae extracts (ASEs) have been investigated for the treatment of various skin disorders. This study reports the effects of ASE-loaded nanoemulsions (NE) on the cellular viability, death by necrosis, and migration of immortalized human keratinocytes (HaCaT cell line), as well as the irritant potential through the hen's egg chorioallantoic membrane test (HET-CAM). NE exhibited a polydispersity index above 0.12, with a droplet size of 300 nm, ζ-potential of -40 mV, and content of flavonoids close to 1 mg/mL. No cytotoxicity of the ASE was observed on HaCaT by MTT assay (up to 10 µg/mL). A significant increase of HaCaT viability was observed to NE (up to 5 μg/mL of flavonoids), compared to treatment with the ASE. The necrosis death evaluation demonstrated that only NE did not lead to cell death at all the tested concentrations. The scratch assay demonstrated that NE was able to increase the cell migration at low flavonoid concentrations. Finally, the HET-CAM test proved the non-irritative potential of NE. Overall, the results indicate the potential of the proposed formulations for topical use in wound healing, in view of their promising effects on proliferation and migration in keratinocytes, combined with an indication of the absence of cytotoxicity and non-irritating potential.
菊科植物提取物（ASEs）已被研究用于治疗各种皮肤疾病。本研究报告了负载ASE的纳米乳液（NE）对永生化人角质形成细胞（HaCaT细胞系）的细胞活力、坏死性死亡和迁移的影响，以及通过鸡胚绒毛尿囊膜试验（HET-CAM）评估其刺激潜力。NE的多分散指数高于0.12，液滴大小为300nm，ζ电位为-40mV，类黄酮含量接近1mg/mL。通过MTT法（高达10μg/mL）未观察到ASE对HaCaT细胞具有细胞毒性。与用ASE处理相比，观察到NE（高达5μg/mL类黄酮）可显著提高HaCaT细胞的活力。坏死性死亡评估表明，在所有测试浓度下，只有NE不会导致细胞死亡。划痕试验表明，低类黄酮浓度下NE能够促进细胞迁移。最后，HET-CAM试验证明了NE无刺激潜力。总体而言，鉴于所提出的制剂对角质形成细胞的增殖和迁移具有良好效果，且无细胞毒性和无刺激潜力，结果表明其在伤口愈合局部应用方面具有潜力。
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