Departamento de Microbiología e Inmunología, Universidad Nacional de Río Cuarto, Ruta 36 Km 601, Río Cuarto, CP 5800 Córdoba, Argentina.
Food Chem Toxicol. 2013 Oct;60:463-70. doi: 10.1016/j.fct.2013.08.005. Epub 2013 Aug 12.
Achyrocline satureioides is widely consumed as infusion or aperitif and shows important therapeutic properties. Previously, we reported absence of genotoxicity of cold aqueous extract (CAE) of A. satureioides by Allium test. However, one test cannot predict the genotoxic effects of a substance. Thus, the aim of this work was to investigate cytotoxicity, genotoxicity and apoptotic ability of CAE of A. satureioides. In addition, CAE was chemically characterized. The cytotoxicity was evaluated by Trypan blue and MTT assays. The apoptotic capacity was evaluated by Hoechst staining and DNA fragmentation-analysis. The genotoxicity was studied by comet assay (CA) and micronucleus test. The identification and quantification of flavonoids were performed by HPLC-ESI-MS/MS. The cytotoxicity studies indicated low toxicity of CAE. In addition, CAE did not induce apoptotic effects on human PBMCs. CAE did not show genotoxicity in vitro against Vero cells, at 10-50 μg/mL. CAE did not induce in vivo genotoxic effects, but it showed at high concentrations cytotoxicity by micronucleus assay. CAE presented flavonoids such as quercetin, 3-O-methylquercetin and luteolin. In conclusion, A. satureioides at popularly concentrations used, in aperitif or infusion, can be consumed safely because did not show any cytotoxic or genotoxic effects.
旱莲草被广泛用作浸剂或开胃酒，具有重要的治疗特性。以前，我们通过大蒜测试报告了旱莲草的冷水提取物（CAE）没有遗传毒性。然而，一个测试不能预测物质的遗传毒性作用。因此，本工作旨在研究旱莲草 CAE 的细胞毒性、遗传毒性和凋亡能力。此外，还对 CAE 进行了化学表征。通过台盼蓝和 MTT 测定评估细胞毒性。通过 Hoechst 染色和 DNA 片段分析评估凋亡能力。通过彗星试验（CA）和微核试验研究遗传毒性。通过 HPLC-ESI-MS/MS 对类黄酮进行鉴定和定量。细胞毒性研究表明 CAE 的毒性低。此外，CAE 未在人 PBMCs 中诱导凋亡作用。CAE 在 10-50μg/mL 时对 Vero 细胞在体外没有表现出遗传毒性。CAE 没有诱导体内遗传毒性作用，但在微核试验中高浓度时表现出细胞毒性。CAE 呈现出类黄酮，如槲皮素、3-O-甲基槲皮素和木樨草素。总之，旱莲草在开胃酒或浸剂中常用浓度下使用时，可以安全食用，因为没有表现出任何细胞毒性或遗传毒性作用。
