Nursing Department, Cangzhou Central Hospital, Cangzhou City, Hebei Province, China.
Department of Anesthesiology, Cangzhou Central Hospital Brain Branch, Cangzhou City, Hebei Province, China.
Exp Dermatol. 2018 Jul;27(7):779-786. doi: 10.1111/exd.13679.
The ischaemia-reperfusion (I/R)-induced skin lesion has been identified as primary cause of pressure ulcers. To date, attempts to prevent pressure ulcers have not produced a significant improvement. Quercetin, one of the most widely distributed flavonoids in fruits and vegetables, exhibits its antioxidant and anti-inflammatory properties against many diseases, including ischaemic heart disease, atherosclerosis and renal injury. In vitro wound scratch assay was first used to assess the function of quercetin in wounding cell model. Next, animal pressure ulcers model was established with two cycles of I/R. The impact of quercetin in the wound recovery, immune cell infiltration and pro-inflammatory cytokines production was investigated in this model. Mechanistic regulation of quercetin at the wound site was also studied. Quercetin accelerated wound closure in cell scratch assay. Dose-response study suggested 1 μmol/L quercetin for in vivo study. In I/R injury model, quercetin treatment significantly accelerated wound closure, reduced immune cell infiltration and pro-inflammatory cytokines production. Signalling study showed quercetin treatment inhibited MAPK but not NFĸB activation. Quercetin treatment improved the wound healing process in I/R lesions by suppressing MAPK pathway. Our results supported that quercetin could be a potential therapeutic agent for pressure ulcers.
缺血再灌注（I/R）诱导的皮肤损伤已被确定为压疮的主要原因。迄今为止，预防压疮的尝试并未取得显著改善。槲皮素是水果和蔬菜中分布最广的类黄酮之一，具有抗氧化和抗炎特性，可预防多种疾病，包括缺血性心脏病、动脉粥样硬化和肾损伤。体外划痕实验首先用于评估槲皮素在创伤细胞模型中的功能。接下来，用两轮 I/R 建立动物压疮模型。在该模型中研究了槲皮素对伤口恢复、免疫细胞浸润和促炎细胞因子产生的影响。还研究了槲皮素在伤口部位的机制调节。槲皮素在细胞划痕实验中加速了伤口闭合。剂量反应研究表明，1 μmol/L 的槲皮素适用于体内研究。在 I/R 损伤模型中，槲皮素治疗可显著加速伤口闭合，减少免疫细胞浸润和促炎细胞因子的产生。信号研究表明，槲皮素抑制 MAPK 但不抑制 NFĸB 激活。槲皮素通过抑制 MAPK 通路改善 I/R 病变中的伤口愈合过程。我们的结果支持槲皮素可能是治疗压疮的潜在治疗剂。
