Pena-Rodríguez Eloy, Mata-Ventosa Aida, Garcia-Vega Laura, Pérez-Torras Sandra, Fernández-Campos Francisco
Topical & Oral Development R+D Reig Jofre Laboratories, 08970 Sant Joan Despi, Spain.
Molecular Pharmacology and Experimental Therapeutics, Department of Biochemistry and Molecular Biomedicine, Institute of Biomedicine, University of Barcelona (IBUB), 08028 Barcelona, Spain.
Pharmaceutics. 2021 Aug 23;13(8):1322. doi: 10.3390/pharmaceutics13081322.
Dexamethasone-loaded polymer hybrid nanoparticles were developed as a potential tool to treat alopecia areata due to their follicular targeting ability. Freeze drying (FD) is a common technique used to improve nanoparticle stability; however, there are few studies focused on its effect on ethyl cellulose lipid-core nanoparticles. Nanoparticles were lyophilized with different cryoprotectants. Sucrose was selected because it allowed for a good resuspension and provided acceptable physicochemical parameters (374.33 nm, +34.7 mV, polydispersion 0.229%, and 98.87% encapsulation efficiency). The nanoparticles obtained were loaded into a pleasant xanthan gum hydrogel, and the rheological, release, and skin permeation profiles of different formulations were studied. The FD formulation significantly modified the particle size, and the drug release and permeation properties were also altered. In addition, analyses of the cytotoxicity and anti-inflammatory efficacy of FD and non-FD particles on human keratinocytes indicated no differences.
负载地塞米松的聚合物杂化纳米颗粒因其对毛囊的靶向能力而被开发为治疗斑秃的潜在工具。冷冻干燥（FD）是一种用于提高纳米颗粒稳定性的常用技术；然而，很少有研究关注其对乙基纤维素脂质核纳米颗粒的影响。纳米颗粒用不同的冷冻保护剂进行冻干。选择蔗糖是因为它能实现良好的再悬浮，并提供可接受的物理化学参数（374.33 nm、+34.7 mV、多分散性0.229%和包封效率98.87%）。将得到的纳米颗粒载入宜人的黄原胶水凝胶中，并研究了不同制剂的流变学、释放和皮肤渗透特性。FD制剂显著改变了粒径，药物释放和渗透特性也发生了改变。此外，对FD和非FD颗粒对人角质形成细胞的细胞毒性和抗炎功效分析表明没有差异。
