Liaoning Ocean and Fisheries Science Research Institute, Liaoning Academy of Agricultural Sciences, Dalian, Liaoning, 116023, P. R. China.
Tianjin Key Laboratory for Modern Drug Delivery & High-Efficiency, School of Pharmaceutical Science and Technology, Tianjin University, Tianjin, P. R. China.
J Sep Sci. 2022 Jan;45(1):172-184. doi: 10.1002/jssc.202100295. Epub 2021 Sep 8.
The separation and purification of biomacromolecules such as nucleic acid is a perpetual topic in separation processes and bioengineering (fine chemicals, biopharmaceutical engineering, diagnostics, and biological characterization). In principle, the solid-phase extraction for nucleic acid exhibits efficient phase separation, low pollution risk, and small sample demand, compared to the conventional liquid-phase extraction. Herein, solid-phase extraction methods are systematically reviewed to outline research progress and explore additional solid-phase sorbents and devices for novel, flexible, and high-efficiency nucleic acid separation processes. The functional materials capture nucleic acid, magnetic and magnetic-free solid-phase extraction methods, separation device design and optimization, and high-throughput automatable applications based on high-performance solid-phase extraction are summarized. Finally, the current challenges and promising topics are discussed.
生物大分子如核酸的分离和纯化是分离过程和生物工程（精细化学品、生物制药工程、诊断和生物特性）中的永恒主题。原则上，与传统的液相萃取相比，核酸固相萃取具有高效的相分离、低污染风险和小的样品需求。在此，系统地综述了固相萃取方法，以概述研究进展，并探索新型、灵活和高效的核酸分离过程的其他固相吸附剂和装置。总结了功能材料捕获核酸、磁固相萃取和无磁固相萃取方法、分离装置设计和优化以及基于高性能固相萃取的高通量自动化应用。最后，讨论了当前的挑战和有前景的课题。
