College of Horticulture and Plant Protection, Institute of Applied Entomology, Yangzhou University, Yangzhou 225009, China.
College of Horticulture and Plant Protection, Institute of Applied Entomology, Yangzhou University, Yangzhou 225009, China; Joint International Research Laboratory of Agriculture and Agri-Product Safety, The Ministry of Education, Yangzhou University, Yangzhou, China.
Comp Biochem Physiol Part D Genomics Proteomics. 2021 Dec;40:100903. doi: 10.1016/j.cbd.2021.100903. Epub 2021 Aug 20.
Chilo suppressalis Walker is a devastating pest of rice in Asia and exhibits facultative diapause in the larval stage. Most prior experiments on diapausing and non-diapausing C. suppressalis were conducted in the laboratory. In this study, transcriptome analyses were performed on pre-diapausing larvae collected from field populations of C. suppressalis and compared to laboratory populations. Among 2674 differentially expressed genes (DEGs), 32 DEGs related to pre-diapause and 239 universally expressed genes were screened; these were primarily enriched in "neuroactive ligand-receptor interaction", "lysosome" and "glycerolipid metabolism" in KEGG pathway analysis. With respect to clusters of orthologous genes (COG), DEGs were assigned to "posttranslational modification, protein turnover, chaperones", "carbohydrate transport and metabolism", and "secondary metabolite biosynthesis, transport and catabolism" categories. Further analysis also revealed that a key "circadian clock-controlled protein" gene is sensitive to photoperiod and significantly decreased during the pre-diapause phase. Genes encoding two small heat shock proteins, hsp21.4 and hsp27.2, were significantly expressed on August 15 as compared to three other sampling times in August 2018. Eight DEGs were randomly chosen and evaluated by real-time quantitative PCR (RT-qPCR) to validate the accuracy of the transcriptome data. The expression of six DEGs (gene-evm_000752, gene-evm_006486, gene-evm_008626, gene-evm_002485, gene-evm_011981 and Chilo_suppressalis_newGene_18103) showed significant same patterns of differential expression in both the RT-qPCR and RNA-Seq analyses. This study increases our understanding of the complex physiological and molecular mechanisms involved in C. suppressalis at the pre-diapause phase.
二化螟是亚洲水稻的毁灭性害虫，其幼虫期具有兼性滞育特性。先前关于滞育和非滞育二化螟的大多数实验都是在实验室进行的。在这项研究中，对来自田间种群的预滞育幼虫和实验室种群的二化螟进行了转录组分析。在 2674 个差异表达基因（DEGs）中，筛选到 32 个与预滞育相关和 239 个普遍表达的基因；KEGG 通路分析主要富集在“神经活性配体-受体相互作用”、“溶酶体”和“甘油脂代谢”。就同源基因簇（COG）而言，DEGs 被分配到“翻译后修饰、蛋白质周转、伴侣”、“碳水化合物运输和代谢”和“次生代谢物生物合成、运输和分解代谢”类别。进一步的分析还表明，一个关键的“生物钟控制蛋白”基因对光周期敏感，在预滞育阶段显著下降。与 2018 年 8 月的其他三个采样时间相比，编码两个小热休克蛋白 hsp21.4 和 hsp27.2 的基因在 8 月 15 日显著表达。随机选择 8 个 DEGs 并通过实时定量 PCR（RT-qPCR）进行评估，以验证转录组数据的准确性。在 RT-qPCR 和 RNA-Seq 分析中，有 6 个 DEGs（基因-evm_000752、基因-evm_006486、基因-evm_008626、基因-evm_002485、基因-evm_011981 和 Chilo_suppressalis_newGene_18103）的表达模式显著相同。这项研究增加了我们对二化螟预滞育阶段复杂生理和分子机制的理解。
