Lu Ming-Xing, Cao Shuang-Shuang, Du Yu-Zhou, Liu Zhong-Xian, Liu Pingyang, Li Jianyong
1] College of Horticulture and Plant Protection & Institute of Applied Entomology, Yangzhou University, Yangzhou, China [2] Department of Biochemistry, Virginia Tech, Blacksburg, VA, United States.
Sci Rep. 2013 Nov 14;3:3211. doi: 10.1038/srep03211.
Diapause is a complex and dynamic process. Chilo suppressalis, an important rice pest in Asia enters facultative diapause as larvae. Our results demonstrated in Yangzhou, China, diapause was initiated between September 4 and 12, 2010. After diapause termination, C. suppressalis remained in quiescence in the field for as long as three months. The average time between collection of field larvae of C. suppressalis and their pupation decreased as the season progressed from fall to next spring. Unexpectedly, the pupated ratio of female to male in the initiation of diapause was 0.22. The abundance of hsp90, hsp70, hsp60 and CsAQP1 all peaked on January 8 or 15, 2011. Nitric oxide (NO) is a secondary messenger that is positively correlated with the diapause of C. suppressalis. Among several geographically separated populations of C. suppressalis, there are no significant differences in the mRNA levels of hsp70, hsp60 or CsAQP1.
滞育是一个复杂且动态的过程。二化螟是亚洲一种重要的水稻害虫，其幼虫进入兼性滞育状态。我们在中国扬州的研究结果表明，滞育于2010年9月4日至12日开始。滞育结束后，二化螟在田间静止状态持续长达三个月。随着季节从秋季向次年春季推进，二化螟田间幼虫采集到化蛹的平均时间缩短。出乎意料的是，滞育开始时雌虫与雄虫的化蛹比例为0.22。热休克蛋白90、热休克蛋白70、热休克蛋白60和CsAQP1的丰度均在2011年1月8日或15日达到峰值。一氧化氮（NO）是一种第二信使，与二化螟的滞育呈正相关。在几个地理隔离的二化螟种群中，热休克蛋白70、热休克蛋白60或CsAQP1的mRNA水平没有显著差异。
