College of Life Science and Engineering, Foshan University, Foshan, Guangdong 528231, People's Republic of China.
College of Life Science and Engineering, Foshan University, Foshan, Guangdong 528231, People's Republic of China.
Int Immunopharmacol. 2021 Nov;100:108079. doi: 10.1016/j.intimp.2021.108079. Epub 2021 Aug 27.
Mastitis is an inflammation of mammary gland, which directly affects the milk production performance and causes huge economic losses in the dairy industry. During mastitis, the blood-milk barrier (BMB) loses its integrity and aggravates the severity of mastitis. Exogenous DNase I has been exerted protective effects in different model of tissue injury. Here, we designed a study to investigate the effects of DNase I on inflammation and BMB in a mice model of Staphylococcus aureus-induced mastitis. In the model, we found that DNase I treatment significantly alleviated the inflammatory response through decrease of inflammatory cells in mammary alveoli, MPO activity and cytokines in mammary gland. Furthermore, immunofluorescent staining and western blotting demonstrated that exogenous DNase I obviously reduced BMB permeability and changed the expression of tight junction proteins to support the re-establishment of the barrier integrity. Mechanismly, DNase I treatment inhibited NF-κB and enhanced AKT signaling pathways. Therefore, our results indicate that DNase I may be an effective treatment for attenuating mastitis.
乳腺炎是乳腺的炎症，直接影响产奶性能，并给奶牛养殖业造成巨大的经济损失。在乳腺炎期间，血乳屏障（BMB）失去完整性，使乳腺炎加重。外源性的 DNA 酶 I 在不同的组织损伤模型中发挥了保护作用。在这里，我们设计了一项研究来调查 DNA 酶 I 对金黄色葡萄球菌诱导的乳腺炎小鼠模型中炎症和 BMB 的影响。在该模型中，我们发现 DNA 酶 I 通过减少乳腺肺泡中的炎性细胞、MPO 活性和细胞因子来显著减轻炎症反应。此外，免疫荧光染色和 Western blot 表明，外源性 DNA 酶 I 明显降低了 BMB 的通透性，并改变了紧密连接蛋白的表达，以支持屏障完整性的重建。机制上，DNA 酶 I 处理抑制 NF-κB 并增强 AKT 信号通路。因此，我们的结果表明 DNA 酶 I 可能是一种有效的治疗乳腺炎的方法。
