The Key Laboratory of Animal Disease and Human Health of Sichuan Province, The Medical Research Center for Cow Disease, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China.
Guangxi Innovates Medical Technology Co., Ltd., Lipu, 546600, Guangxi, China.
Sci Rep. 2022 Nov 5;12(1):18829. doi: 10.1038/s41598-022-23749-x.
Intramuscular injection of anemoside B4 (AB4) has a superior therapeutic effect on clinical mastitis in lactating cows. Here, we explored AB4's effect on milk whey in clinical mastitis-affected cows using proteomics. Among fifty clinical mastitis cows received AB4 administration (0.05 ml/kg/day, for 7 days), twelve healed cows were selected and marked as group T. Twelve clinically heathy cows received the same dose of saline for 7 days, marked as group C. Collected milk whey of group T before and after AB4 administration marked as T1 and T2, respectively. The milk whey of group C after saline injection marked as C1. Milk whey protein changes were detected using tandem mass tag-based quantitative proteomic. We identified 872 quantifiable proteins in the samples. Among them, 511 proteins between T1 and C1, and 361 proteins between T2 and T1 were significantly altered. T1 than C1 had significantly more proteins associated with inflammatory damage and trans-endothelial migration of leukocytes, whereas these proteins were reduced in T2 treated with AB4. Compared with C, proteins associated with fibrin clot degradation and complement system activation were downregulated in T1 but upregulated in T2. In summary, AB4 can exert its therapeutic effect on clinical mastitis in cows mainly by reducing inflammatory damage, activating the complement system, inhibiting trans-endothelial migration of leukocytes, and promoting degradation of milk fibrin clots.
肌肉内注射川陈皮素（AB4）对哺乳期奶牛临床乳腺炎具有更好的治疗效果。在这里，我们使用蛋白质组学探索了 AB4 对临床乳腺炎奶牛乳清的影响。在接受 AB4 治疗（0.05ml/kg/天，连续 7 天）的五十头临床乳腺炎奶牛中，选择了十二头治愈的奶牛并标记为 T 组。十二头临床健康的奶牛接受相同剂量的生理盐水治疗 7 天，标记为 C 组。分别收集 T 组在 AB4 给药前后的乳清，标记为 T1 和 T2。C 组在生理盐水注射后的乳清标记为 C1。使用串联质量标签定量蛋白质组学检测乳清蛋白变化。在样品中鉴定出 872 种可定量的蛋白质。其中，T1 与 C1 之间有 511 种蛋白质，T2 与 T1 之间有 361 种蛋白质发生显著变化。T1 与 C1 相比，与炎症损伤和白细胞跨内皮迁移相关的蛋白质明显更多，而在 AB4 治疗的 T2 中这些蛋白质减少。与 C 相比，T1 中与纤维蛋白凝块降解和补体系统激活相关的蛋白质下调，但 T2 中上调。总之，AB4 可以通过减轻炎症损伤、激活补体系统、抑制白细胞跨内皮迁移和促进乳纤维蛋白凝块降解来发挥对奶牛临床乳腺炎的治疗作用。
