Lisak V M, Korolev M B, Dzhivanian T I, Karganova G G, Lashkevich V A
Vopr Virusol. 1987 Nov-Dec;32(6):696-701.
Ultrastructural features of tick-borne encephalitis virus-infected pig embryo cell culture treated with different concentrations of tunicamycin at various intervals after infection were studied electron microscopically. The inhibition of glycosylation did not prevent virion formation in the infected cells. At the same time, treatment with tunicamycin led to marked accumulation of virus particles in cisterns and vacuoles of the Golgi complex and to a decrease in the number of virions released into the extracellular space. It is assumed that inhibition of glycosylation leads to disorders in the regulation of the final stages of virus particles transportation and release from the infected cell. The results of the study indicate an important role of the Golgi complex in realization of the final stages of flavivirus morphogenesis.
用电子显微镜研究了在感染蜱传脑炎病毒后的不同时间间隔，用不同浓度衣霉素处理的猪胚胎细胞培养物的超微结构特征。糖基化的抑制并不妨碍感染细胞中病毒粒子的形成。与此同时，衣霉素处理导致病毒粒子在高尔基体的池和液泡中显著积累，并导致释放到细胞外空间的病毒粒子数量减少。据推测，糖基化的抑制导致病毒粒子从感染细胞运输和释放的最后阶段的调节紊乱。该研究结果表明高尔基体在黄病毒形态发生最后阶段的实现中起重要作用。
