Maleki Shayan, Farhadi Mohammad, Kamrava Seyed Kamran, Asghari Alimohamad, Daneshi Ahmad
PhD, ENT and Head & Neck Research Center and Department, Hazrat Rasoul Hospital, the Five Senses Institute, Iran University of Medical Sciences, Tehran, Iran.
MD, ENT and Head & Neck Research Center and Department, Hazrat Rasoul Hospital, the Five Senses Institute, Iran University of Medical Sciences, Tehran, Iran.
J Biomed Phys Eng. 2021 Aug 1;11(4):435-446. doi: 10.31661/jbpe.v0i0.1108. eCollection 2021 Aug.
Selective targeting of malignant cells is the ultimate goal of anticancer studies around the world. There are some modalities for cancer therapy devastating tumor size and growth rate, meanwhile attacking normal cells. Utilizing appropriate ligands, like folate, allow the delivery of therapeutic molecules to cancer cells selectively. There are a variety of photosensitizers, like gold nanorods (GNRs), capable of absorbing the energy of light and converting it to heat, evidently build a photothermal procedure for cancer therapy.
To develop a one-step approach for calculating the temperature distribution by solving the heat transfer equation with multiple heat sources originating from NIR laser-exposed GNRs.
In this experimental study, we simulated NIR laser heating process in a single cancer cell, with and without incubation with folate conjugated PEG-GNRs. This simulation was based on a real TEM image from an experiment with the same setup. An experiment based on aforesaid scenario was performed to validate the simulated model in practice.
According to the simplifications due to computational resource limits, the resulting outcome of simulation showed significant compatibility to the supporting experiment. Both simulation and experimental studies showed a similar trend for heating and cooling of the cells incubated with GNRs and irradiated by NIR laser (5 min, 1.8 W/cm). It was observed that temperature of the cells in microplate reached 53.6 °C when irradiated by laser.
This new method can be of great application in developing a planning technique for treating tumors utilizing GNP-mediated thermal therapy.
选择性靶向恶性细胞是全球抗癌研究的最终目标。目前有一些癌症治疗方法在破坏肿瘤大小和生长速率的同时，也会攻击正常细胞。利用合适的配体，如叶酸，可使治疗分子选择性地递送至癌细胞。有多种光敏剂，如金纳米棒（GNRs），能够吸收光能并将其转化为热能，显然为癌症治疗构建了一种光热疗法。
开发一种通过求解源自近红外激光照射的金纳米棒的多热源热传递方程来计算温度分布的一步法。
在本实验研究中，我们模拟了单个癌细胞在有无叶酸共轭聚乙二醇 - 金纳米棒孵育情况下的近红外激光加热过程。该模拟基于相同设置实验的真实透射电子显微镜图像。基于上述情况进行了一项实验，以在实际中验证模拟模型。
由于计算资源限制进行简化后，模拟结果与支持实验显示出显著的兼容性。模拟和实验研究均显示，用金纳米棒孵育并用近红外激光（5分钟，1.8W/cm）照射的细胞的加热和冷却趋势相似。观察到当用激光照射时，微孔板中细胞的温度达到53.6°C。
这种新方法在开发利用金纳米颗粒介导的热疗法治疗肿瘤的规划技术方面具有很大的应用价值。
