Department of Medical Physics and Medical Engineering, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran; Ionizing and Non-ionizing Radiation Protection Research Center (INIRPRC), Shiraz University of Medical Sciences, Shiraz, Iran.
Medical Physics Department, School of Medicine, Iran University of Medical Sciences (IUMS), Tehran, Iran.
Photodiagnosis Photodyn Ther. 2018 Dec;24:324-331. doi: 10.1016/j.pdpdt.2018.10.016. Epub 2018 Oct 27.
In this article, we report a targeted cancer treatment strategy using nano-photo-thermal therapy (NPTT) and radiotherapy (RT) methods. Gold nanorods (AuNRs) without and with folic acid (FA) conjugation were firstly synthesized and then characterized. Cytotoxicity of various methods; NPTT (nanoparticles; 808 nm laser; 2 W/cm) or RT (6 MV X-ray; 2 Gy) or combination of NPTT and RT; was separately investigated on KB nasopharyngeal carcinoma cells. The effects of different treatments were studies using MTT assay, inductively coupled plasma-mass spectrometry (ICP-MS) and transmission electron microscopy (TEM). Both TEM and ICP-MS confirmed the internalization of nanoparticles into the KB cells. ICP-MS analysis revealed that AuNR-FA cell uptake was higher than AuNRs. Viability of the cells received NPTT was lower than those cells received laser or nanoparticles alone. Furthermore, it was found that combination of NPTT and RT notably decreased the viability of KB cells. Following such a combinatorial treatment (NPTT + RT), intensive damages were identified in TEM images obtained from treated cells. It may be concluded that AuNR-FA nanoconjugate is a good candidate to be used as a targeted sensitizer agent for nano-photo-thermo-radiotherapy of head and neck cancer cells.
在本文中，我们报告了一种使用纳米光热疗法（NPTT）和放射疗法（RT）的靶向癌症治疗策略。首先合成了没有和有叶酸（FA）缀合的金纳米棒（AuNRs），然后对其进行了表征。分别研究了 NPTT（纳米颗粒；808nm 激光；2W/cm）或 RT（6MV X 射线；2Gy）或 NPTT 和 RT 联合的各种方法的细胞毒性；通过 MTT 测定、电感耦合等离子体质谱（ICP-MS）和透射电子显微镜（TEM）研究了不同处理方法的效果。TEM 和 ICP-MS 均证实了纳米颗粒进入 KB 鼻咽癌细胞。ICP-MS 分析表明，AuNR-FA 细胞摄取量高于 AuNRs。接受 NPTT 的细胞活力低于单独接受激光或纳米颗粒的细胞活力。此外，还发现 NPTT 和 RT 的联合显著降低了 KB 细胞的活力。在这种联合治疗（NPTT+RT）后，从处理过的细胞中获得的 TEM 图像中可以识别出密集的损伤。可以得出结论，AuNR-FA 纳米复合物是作为头颈癌细胞的纳米光热放射治疗的靶向敏化剂的良好候选物。
