Gereis M, Burford-Mason A P, Watkins S M
Department of Pathology, Lister Hospital, Stevenage, Herts, U.K.
Agents Actions. 1987 Dec;22(3-4):324-9. doi: 10.1007/BF02009063.
Cultures of normal donor peripheral blood mononuclear cells were tested in vitro for suppression by chemotherapeutic agents or their metabolites. The drugs tested were those commonly used in the treatment of breast cancer, namely, 5-fluorouracil, doxorubicin, vincristine, methotrexate and cyclophosphamide (actually testing its active metabolite, 4-hydroxy-cyclophosphamide). The lymphocytes were stimulated by phytohaemagglutinin (PHA), and the inhibitory effect of the drugs on subsequent DNA synthesis was measured by tritiated thymidine uptake. Drug concentrations used were equivalent to expected in vivo plasma and body fluid levels following i.v. injection of a standard therapeutic dose. Results suggest that the drugs may be ranked for suppression of T-cell function as follows: doxorubicin greater than vincristine = cyclophosphamide greater than 5-fluorouracil greater than methotrexate.
对正常供体的外周血单个核细胞培养物进行体外测试，以检测化疗药物或其代谢产物的抑制作用。所测试的药物为常用于治疗乳腺癌的药物，即5-氟尿嘧啶、阿霉素、长春新碱、甲氨蝶呤和环磷酰胺（实际上测试的是其活性代谢产物4-羟基环磷酰胺）。淋巴细胞用植物血凝素（PHA）刺激，通过氚标记胸腺嘧啶核苷摄取来测量药物对后续DNA合成的抑制作用。所使用的药物浓度相当于静脉注射标准治疗剂量后体内血浆和体液中的预期水平。结果表明，这些药物对T细胞功能的抑制作用排序如下：阿霉素＞长春新碱=环磷酰胺＞5-氟尿嘧啶＞甲氨蝶呤。
