猪不同细胞系基因表达研究中参考基因的验证。
Validation of the Reference Genes for the Gene Expression Studies in Different Cell Lines of Pig.
机构信息
State Key Laboratory of Animal Nutrition; Key Laboratory of Animal Genetics Breeding and Reproduction, Ministry of Agriculture and Rural Affairs, Institute of Animal Science, Chinese Academy of Agricultural Sciences, 100193 Beijing, China.
Guangdong Provincial Key Laboratory of Animal Molecular Design and Precise Breeding, College of Life Science and Engineering, Foshan University, 528231 Foshan, China.
出版信息
Biomed Res Int. 2021 Aug 18;2021:5364190. doi: 10.1155/2021/5364190. eCollection 2021.
Reverse transcription quantitative real-time polymerase chain reaction is one of the important methods to investigate gene expression in cells and tissues. However, if the data cannot be normalized with appropriate reference genes, the results may be unreliable. In this study, we detected the expression of 15 reference genes in three pig cell lines. The results showed that and were the most stable reference genes in 3D4/21 cells. , , and were the most stable reference genes in PK-15 cells. and were the most stable reference genes in IPEC-J2 cells. In addition, each cell line only needs to use two reference genes to standardize the expression of target genes. Taken together, this study provides a reference for different pig cell lines to select reference genes and also provides a theoretical basis for the use of these cell lines in related functional researches.
逆转录定量实时聚合酶链反应是研究细胞和组织中基因表达的重要方法之一。然而，如果不能用适当的参照基因对数据进行标准化，结果可能不可靠。本研究检测了三种猪细胞系中 15 个参照基因的表达情况。结果表明，在 3D4/21 细胞中， 和 是最稳定的参照基因。在 PK-15 细胞中， 、 和 是最稳定的参照基因。在 IPEC-J2 细胞中， 和 是最稳定的参照基因。此外，每种细胞系只需要使用两个参照基因来标准化目的基因的表达。综上所述，本研究为不同的猪细胞系选择参照基因提供了参考，也为这些细胞系在相关功能研究中的应用提供了理论依据。
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