Gong Xiaocheng, Li Xuepeng, You Xin, Hu Aoxue, Liu Min, Yao Huimin, He Jun, Zhang Xianghan, Ning Pengbo
School of Life Science and Technology, Xidian University, Xi'an, Shaanxi, China.
Engineering Research Center of Molecular & Neuroimaging, Ministry of Education, Xi'an, Shaanxi, China.
PeerJ. 2020 Feb 17;8:e8543. doi: 10.7717/peerj.8543. eCollection 2020.
Classical swine fever (CSF) is a disease that is characterized by diffuse hemorrhaging, high fever, and high mortality rates. The pro-inflammatory characteristics of allograft inflammatory factor 1 (AIF1) have been well documented; however, insufficient attention has been given to porcine AIF1. In the present study, AIF1 was identified as a key player contributing to CSFV Shimen infection in porcine alveolar macrophage (PAM) 3D4/21 cell line. Our evaluation showed that AIF1 mRNA and protein are expressed at a time-dependent high level in CSFV Shimen-infected PAM 3D4/21 cells. The transcription and translation of IL6 were also significantly upregulated in infected PAM 3D4/21 cells. By utilizing overexpression RNAs approach, we showed that the cellular AIF1 induced an increased IL6 in PAM 3D4/21 cells. Furthermore, silencing of AIF1 suppressed CSFV Shimen-induced IL6 production in PAM 3D4/21 cells and also inhibited CSFV replication, whereas overexpression of recombinant AIF1 was beneficial for the replication of CSFV Shimen and promoting IL6 production in CSFV Shimen-infected PAM 3D4/21 cells. It is suggested CSFV Shimen induced IL6 in PAM 3D4/21 cells via AIF1 activation, which help clarify the AIF1-related inflammatory processes that occur on CSFV Shimen infected macrophages.
经典猪瘟（CSF）是一种以弥漫性出血、高热和高死亡率为特征的疾病。同种异体移植炎症因子1（AIF1）的促炎特性已有充分记载；然而，猪AIF1却未得到足够关注。在本研究中，AIF1被确定为在猪肺泡巨噬细胞（PAM）3D4/21细胞系中促成猪瘟病毒石门株（CSFV Shimen）感染的关键因素。我们的评估显示，在CSFV Shimen感染的PAM 3D4/21细胞中，AIF1 mRNA和蛋白呈时间依赖性高水平表达。感染的PAM 3D4/21细胞中白细胞介素6（IL6）的转录和翻译也显著上调。通过采用RNA过表达方法，我们发现细胞内AIF1可使PAM 3D4/21细胞中的IL6增加。此外，敲低AIF1可抑制CSFV Shimen诱导的PAM 3D4/21细胞中IL6的产生，还能抑制CSFV的复制，而重组AIF1的过表达则有利于CSFV Shimen的复制，并促进CSFV Shimen感染的PAM 3D4/21细胞中IL6的产生。提示CSFV Shimen通过激活AIF1在PAM 3D4/21细胞中诱导IL6产生，这有助于阐明CSFV Shimen感染巨噬细胞时发生的与AIF1相关的炎症过程。
