小鼠脑片黑质致密部多巴胺能神经元的细胞贴附式和全细胞膜片钳记录
Cell-attached and Whole-cell Patch-clamp Recordings of Dopamine Neurons in the Substantia Nigra Pars Compacta of Mouse Brain Slices.
作者信息
Cattaneo Stefano, Regoni Maria, Sassone Jenny, Taverna Stefano
机构信息
Vita-Salute San Raffaele University, Milan, Italy.
Division of Neuroscience, San Raffaele Scientific Institute, Milan, Italy.
出版信息
Bio Protoc. 2021 Aug 5;11(15):e4109. doi: 10.21769/BioProtoc.4109.
Abstract
The Substantia Nigra pars compacta (SNc) is a midbrain dopaminergic nucleus that plays a key role in modulating motor and cognitive functions. It is crucially involved in several disorders, particularly Parkinson's disease, which is characterized by a progressive loss of SNc dopaminergic cells. Electrophysiological studies on SNc neurons are of paramount importance to understand the role of dopaminergic transmission in health and disease. Here, we provide an extensive protocol to prepare SNc-containing mouse brain slices and record the electrical activity of dopaminergic cells. We describe all the necessary steps, including mouse transcardiac perfusion, brain extraction, slice cutting, and patch-clamp recordings.
摘要
黑质致密部（SNc）是中脑多巴胺能核团，在调节运动和认知功能中起关键作用。它与多种疾病密切相关，尤其是帕金森病，其特征是SNc多巴胺能细胞逐渐丧失。对SNc神经元的电生理研究对于理解多巴胺能传递在健康和疾病中的作用至关重要。在这里，我们提供了一个详细的方案来制备包含SNc的小鼠脑片并记录多巴胺能细胞的电活动。我们描述了所有必要步骤，包括小鼠经心灌注、脑提取、切片以及膜片钳记录。
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