Cox Thomas R, Madsen Chris D
The Garvan Institute of Medical Research and The Kinghorn Cancer Centre, Cancer Division, St Vincent's Clinical School, Faculty of Medicine, University of New South Wales, Sydney, Australia.
Department of Laboratory Medicine, Division of Translational Cancer Research, Lund University, Lund, Sweden.
Bio Protoc. 2017 Jan 5;7(1):e2101. doi: 10.21769/BioProtoc.2101.
Hydrogel systems composed of purified extracellular matrix (ECM) components (such as collagen, fibrin, Matrigel, and methylcellulose) are a mainstay of cell and molecular biology research. They are used extensively in many applications including tissue regeneration platforms, studying organ development, and pathological disease models such as cancer. Both the biochemical and biomechanical properties influence cellular and tissue compatibility, and these properties are altered in pathological disease progression (Cox and Erler, 2011; Bonnans , 2014 ). The use of cell-embedded hydrogels in disease models such as cancer, allow the interrogation of cell-induced changes in the biomechanics of the microenvironment ( Madsen , 2015 ). Here we report a simple method to measure these cell-induced changes using a controlled strain rotational rheometer.
由纯化的细胞外基质（ECM）成分（如胶原蛋白、纤维蛋白、基质胶和甲基纤维素）组成的水凝胶系统是细胞和分子生物学研究的支柱。它们广泛应用于许多领域，包括组织再生平台、研究器官发育以及癌症等病理疾病模型。生化和生物力学特性都会影响细胞和组织的相容性，并且这些特性在病理疾病进展过程中会发生改变（考克斯和埃勒，2011年；博南斯，2014年）。在癌症等疾病模型中使用细胞嵌入水凝胶，可以探究细胞诱导的微环境生物力学变化（马德森，2015年）。在此，我们报告一种使用可控应变旋转流变仪测量这些细胞诱导变化的简单方法。
