……中的淹没接种法
Flood-inoculation Method in .
作者信息
Ishiga Yasuhiro, Ishiga Takako, Ichinose Yuki, Mysore Kirankumar S
机构信息
Faculty of Life and Environmental Sciences, University of Tsukuba, Tsukuba, Japan.
Laboratory of Plant Pathology and Genetic Engineering, Graduate School of Environmental and Life Science, Okayama University, Okayama, Japan.
出版信息
Bio Protoc. 2017 Jan 20;7(2):e2106. doi: 10.21769/BioProtoc.2106.
Abstract
pv. strain DC3000 ( DC3000), which causes bacterial speck disease of tomato, has been used as a model pathogen because of its pathogenicity on . Here, we demonstrate a rapid and reliable flood-inoculation method based on young seedlings grown on one-half strength MS medium. We also describe a method to evaluate the bacterial growth in .
摘要
可引发番茄细菌性斑点病的丁香假单胞菌番茄致病变种DC3000（DC3000），因其致病性而被用作模式病原体。在此，我们展示了一种基于在半强度MS培养基上生长的幼苗的快速且可靠的淹没接种方法。我们还描述了一种评估[具体部位]细菌生长的方法。
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