在拟南芥中异源表达中国野生葡萄 VqERFs 增强了对丁香假单胞菌 pv. 番茄 DC3000 和灰葡萄孢的抗性。

Heterologous expression of Chinese wild grapevine VqERFs in Arabidopsis thaliana enhance resistance to Pseudomonas syringae pv. tomato DC3000 and to Botrytis cinerea.

机构信息

College of Horticulture, Northwest A & F University, Yangling, Shaanxi 712100, China; Key Laboratory of Horticultural Plant Biology and Germplasm Innovation in Northwest China, Ministry of Agriculture, Yangling, Shaanxi 712100, China; State Key Laboratory of Crop Stress Biology in Arid Areas, Northwest A & F University, Yangling, Shaanxi 712100, China.

出版信息

Plant Sci. 2020 Apr;293:110421. doi: 10.1016/j.plantsci.2020.110421. Epub 2020 Jan 23.

DOI:10.1016/j.plantsci.2020.110421

PMID:32081269

Abstract

When a plant is attacked by a pathogen, an immune response is activated to help protect it from harm. ERF transcription factors have been reported to regulate immune responses in plants. Here, three ERF transcription factors from Chinese wild Vitis quinquangularis, VqERF112, VqERF114 and VqERF072, are shown to respond to pathogen inoculation by powdery mildew, Pseudomonas syringae pv. tomato (Pst) DC3000 and Botrytis cinerea and to hormone treatments including with ET, SA, MeJA or ABA. Tissue specific expression analysis shows the highest expression levels of VqERF112 and VqERF114 were in mature berries and of VqERF072 was in tendrils. A GUS activity assay indicates that the promoters of VqERF112, VqERF114 and VqERF072 can be induced by powdery mildew inoculation and by hormone treatment, including with ET, SA and MeJA. Overexpression of VqERF112, VqERF114 and VqERF072 in transgenic Arabidopsis enhanced the resistance to Pseudomonas syringae pv. tomato DC3000 (Pst DC3000) and B. cinerea, and it increased the expression of the SA signaling-related genes AtNPR1 and AtPR1 and of the JA/ET signaling-related genes AtPDF1.2, AtLOX3, AtPR3 and AtPR4. Compared to Col-0 plants, the HO accumulation in transgenic Arabidopsis increased after Pst DC3000 inoculation but decreased after B. cinerea inoculation. These results demonstrate that VqERF112, VqERF114 and VqERF072 positively regulate resistance to Pst DC3000 and B. cinerea.

摘要

当植物受到病原体攻击时，会激活免疫反应来帮助它免受伤害。已报道 ERF 转录因子调节植物的免疫反应。在这里，从中国野生五倍子中分离的三个 ERF 转录因子，VqERF112、VqERF114 和 VqERF072，被证明可以对白粉病、丁香假单胞菌 pv.番茄（Pst）DC3000 和灰霉病菌的接种以及包括 ET、SA、MeJA 或 ABA 在内的激素处理做出反应。组织特异性表达分析表明，VqERF112 和 VqERF114 的表达水平最高的是成熟浆果，而 VqERF072 的表达水平最高的是卷须。GUS 活性测定表明，VqERF112、VqERF114 和 VqERF072 的启动子可以被白粉病接种和激素处理诱导，包括 ET、SA 和 MeJA。在转基因拟南芥中过表达 VqERF112、VqERF114 和 VqERF072 增强了对丁香假单胞菌 pv.番茄 DC3000（Pst DC3000）和灰霉病菌的抗性，同时增加了 SA 信号相关基因 AtNPR1 和 AtPR1 以及 JA/ET 信号相关基因 AtPDF1.2、AtLOX3、AtPR3 和 AtPR4 的表达。与 Col-0 植物相比，转基因拟南芥在接种 Pst DC3000 后 HO 积累增加，但在接种灰霉病菌后减少。这些结果表明，VqERF112、VqERF114 和 VqERF072 正向调节对 Pst DC3000 和灰霉病菌的抗性。