Lin Wen-Ling, Mizobuchi Mizuki, Kawahigashi Mina, Nakahashi Otoki, Maekawa Yuuki, Sakai Takashi
Institute for Health Sciences, Tokushima Bunri University, 180 Nishihama-bouji, Yamashiro-cho, Tokushima, 770-8514, Japan.
Biochem Biophys Rep. 2021 Aug 17;27:101103. doi: 10.1016/j.bbrep.2021.101103. eCollection 2021 Sep.
We established a method of KC transplantation by intraperitoneal (i.p.) injection using EGFP-expressing cells (EGFP-KCs) and normal KCs. The novel method is easier and less invasive than conventional methods so that it is not only technically advantageous but also ethically preferable for experiments using animals. We demonstrated that KCs migrated to the liver following i.p. Injection. Engraftment in the liver was not observed for peritoneal macrophages (pMPs). This suggests that KCs migrate to the liver via a sorting mechanism. KC injection decreased the KC number at 24 h and then recovered the KCs at 10 days to a normal level. Additionally, recovery to the normal level by KC injection was observed in mice with KC depletion induced by GdCl. These results suggest that a regulatory mechanism exists for controlling the number of KCs.
我们通过腹腔内（i.p.）注射表达增强绿色荧光蛋白的细胞（EGFP-KCs）和正常KCs建立了一种KC移植方法。这种新方法比传统方法更简便且侵入性更小，因此不仅在技术上具有优势，而且在使用动物的实验中在伦理上更可取。我们证明腹腔注射后KCs迁移至肝脏。未观察到腹腔巨噬细胞（pMPs）在肝脏中植入。这表明KCs通过一种分选机制迁移至肝脏。KC注射在24小时时减少了KC数量，然后在10天时将KCs恢复至正常水平。此外，在由GdCl诱导的KC耗竭的小鼠中观察到通过KC注射恢复至正常水平。这些结果表明存在一种控制KCs数量的调节机制。
