枯否细胞对肝移植免疫耐受的影响。

Effect of Kupffer cells on immune tolerance in liver transplantation.

机构信息

Second Xiangya Hospital of Central South University, Changsha 410011, China.

出版信息

Asian Pac J Trop Med. 2012 Dec;5(12):970-2. doi: 10.1016/S1995-7645(12)60184-9.

DOI:10.1016/S1995-7645(12)60184-9

PMID:23199716

Abstract

OBJECTIVE

To observe the effect of Kupffer cells on immune tolerance in liver transplantation.

METHODS

The rats were randomly divided into A, B and C groups. A group was sham operation group. The donor rats of group B had intraperitoneal injection of 1 nmol Kuppffer cells every other day for three days before liver transplantation. Rats of group C were injected with equal saline. The rat liver transplantation models were established by modified Kamada's two-cuff technique. The rats were sacrificed after 24 hours. The concentrations of ALT and AST in serum were measured with the biochemical analyzer. The level of IL-2 and TNF-α in serum were measured by ELISA method. The apoptotic indexes were detected by immunohistochemical assay.

RESULTS

The concentration of ALT, AST, IL-1 and TNF-α in A, B and C groups were increased successively. The levels of group C were significantly higher than that of group B and A (P<0.05), and the levels of group B were significantly higher than that of group A (P<0.05). The apoptotic indexes of three groups were 3.40±0.37, 14.70±2.54 and 26.33±3.65, respectively, with significant difference among three groups (P<0.05).

CONCLUSIONS

Pretreatment with Kupffer cells can reduce liver injury and raise liver transplantation immune tolerance.

摘要

目的

观察枯否细胞对肝移植免疫耐受的影响。

方法

将大鼠随机分为 A、B、C 三组。A 组为假手术组。B 组供体大鼠在肝移植前每隔一天腹腔注射 1nmol 枯否细胞，共 3 天。C 组注射等量生理盐水。采用改良的 Kamada 双袖套技术建立大鼠肝移植模型。大鼠在 24 小时后处死。用生化分析仪检测血清中 ALT 和 AST 的浓度。采用 ELISA 法检测血清中 IL-2 和 TNF-α的水平。免疫组化法检测细胞凋亡指数。

结果

A、B、C 三组的 ALT、AST、IL-1 和 TNF-α浓度依次升高。C 组的水平明显高于 B 组和 A 组（P<0.05），B 组的水平明显高于 A 组（P<0.05）。三组细胞凋亡指数分别为 3.40±0.37、14.70±2.54、26.33±3.65，三组间差异有统计学意义（P<0.05）。