Nassir Nasna, Tambi Richa, Bankapur Asma, Al Heialy Saba, Karuvantevida Noushad, Khansaheb Hamda Hassan, Zehra Binte, Begum Ghausia, Hameid Reem Abdel, Ahmed Awab, Deesi Zulfa, Alkhajeh Abdulmajeed, Uddin K M Furkan, Akter Hosneara, Safizadeh Shabestari Seyed Ali, Almidani Omar, Islam Amirul, Gaudet Mellissa, Kandasamy Richard Kumaran, Loney Tom, Tayoun Ahmad Abou, Nowotny Norbert, Woodbury-Smith Marc, Rahman Proton, Kuebler Wolfgang M, Yaseen Hachim Mahmood, Casanova Jean-Laurent, Berdiev Bakhrom K, Alsheikh-Ali Alawi, Uddin Mohammed
College of Medicine, Mohammed Bin Rashid University of Medicine and Health Sciences, Dubai, UAE.
Meakins-Christie Laboratories, Research Institute of the McGill University Health Center, Montreal, QC, Canada.
iScience. 2021 Sep 24;24(9):103030. doi: 10.1016/j.isci.2021.103030. Epub 2021 Aug 25.
Understanding host cell heterogeneity is critical for unraveling disease mechanism. Utilizing large-scale single-cell transcriptomics, we analyzed multiple tissue specimens from patients with life-threatening COVID-19 pneumonia, compared with healthy controls. We identified a subtype of monocyte-derived alveolar macrophages (MoAMs) where genes associated with severe COVID-19 comorbidities are significantly upregulated in bronchoalveolar lavage fluid of critical cases. consistently demarcated MoAM subset in different samples from severe COVID-19 cohorts and in -upregulated cells from nasopharyngeal swabs. findings were validated by upregulation of in nasopharyngeal swabs of severe ICU COVID-19 cases, particularly in older patients and those with comorbidities. Additional lines of evidence from transcriptomic data and of severe COVID-19 cases suggest that may identify a specific subtype of MoAM in patients with severe COVID-19 that may present a novel biomarker for screening and prognosis, as well as a potential therapeutic target.
了解宿主细胞异质性对于阐明疾病机制至关重要。我们利用大规模单细胞转录组学技术，分析了危及生命的新冠肺炎患者的多个组织样本，并与健康对照进行了比较。我们鉴定出一种单核细胞衍生的肺泡巨噬细胞（MoAM）亚型，在重症病例的支气管肺泡灌洗液中，与严重新冠肺炎合并症相关的基因显著上调。在来自重症新冠肺炎队列的不同样本以及鼻咽拭子中上调的细胞中，始终能划分出MoAM亚群。在重症ICU新冠肺炎病例的鼻咽拭子中，尤其是老年患者和有合并症的患者中，通过[此处原文缺失相关内容]的上调验证了这些发现。来自转录组数据和重症新冠肺炎病例的[此处原文缺失相关内容]的其他证据表明，[此处原文缺失相关内容]可能识别出重症新冠肺炎患者中MoAM的一种特定亚型，这可能为筛查和预后提供一种新的生物标志物，以及一个潜在的治疗靶点。
