Department I of Biliary Tract, Eastern Hepatobiliary Surgery Hospital, Second Military Medical University, Shanghai, China.
The International Cooperation Laboratory on Signal Transduction, Eastern Hepatobiliary Surgery Hospital, Shanghai, China.
Liver Int. 2022 Jan;42(1):135-148. doi: 10.1111/liv.15047. Epub 2021 Sep 3.
BACKGROUND & AIMS: Preoperative obstructive jaundice is usually associated with higher post-operative mortality. Although external biliary drainage (EBD) has been widely used to relieve obstructive jaundice, the role of bile reinfusion after EBD is still controversial. The aim of our study was to study the effects of biliary obstruction, biliary drainage and bile reinfusion on bile acid metabolism and gut microbiota.
Firstly, we created a mice bile drainage collection (BDC) model to simulate the process of biliary obstruction, drainage and bile reinfusion. Then, we analysed the faecal, serum, liver and bile samples to investigate the effects of the process on bile acid profiles and gut microbiota. Finally, we evaluated the clinical effects of bile reinfusion.
We evaluated the bile acid profiles of faeces, serum, liver and bile of normal mice. During biliary obstruction, secondary bile acids can still be produced, and increased in the liver and serum of mice. Compared with no bile reinfusion, bile reinfusion was beneficial to the recovery of T-ωMCA in the liver and bile, and can restore the colon crypt length shortened by biliary obstruction. Only Ruminococcus_1 proliferated when the biliary obstruction lasted for 12 days. In the clinic, bile reinfusion cannot accelerate the patient's perioperative recovery or prolong long-term survival.
We have successfully created a mice bile drainage collection model. Short-term bile reinfusion can partially benefit the recovery of the secondary bile acids in the liver and bile, but hardly benefit the patient's perioperative recovery or long-term survival. (247 words).
术前梗阻性黄疸通常与术后死亡率升高相关。尽管外引流（EBD）已广泛用于缓解梗阻性黄疸，但 EBD 后胆汁再灌注的作用仍存在争议。本研究旨在研究胆汁淤积、胆汁引流和胆汁再灌注对胆汁酸代谢和肠道微生物群的影响。
首先，我们建立了一个小鼠胆汁引流收集（BDC）模型来模拟胆汁淤积、引流和胆汁再灌注的过程。然后，我们分析了粪便、血清、肝脏和胆汁样本，以研究该过程对胆汁酸谱和肠道微生物群的影响。最后，我们评估了胆汁再灌注的临床效果。
我们评估了正常小鼠粪便、血清、肝脏和胆汁的胆汁酸谱。在胆汁淤积期间，仍可产生次级胆汁酸，且小鼠肝脏和血清中的胆汁酸含量增加。与无胆汁再灌注相比，胆汁再灌注有利于 T-ωMCA 在肝脏和胆汁中的恢复，并能恢复胆汁淤积引起的结肠隐窝长度缩短。只有当胆汁淤积持续 12 天时，Ruminococcus_1 才会增殖。在临床上，胆汁再灌注不能加速患者围手术期的恢复或延长长期生存。
我们成功建立了小鼠胆汁引流收集模型。短期胆汁再灌注可以部分有益于肝脏和胆汁中次级胆汁酸的恢复，但对患者围手术期的恢复或长期生存几乎没有益处。（247 个单词）
