Department of Experimental and Clinical Medicine, University of Florence.
Department of Experimental and Clinical Biomedical Sciences "Mario Serio", University of Florence.
Eur J Histochem. 2021 Aug 13;65(s1):3282. doi: 10.4081/ejh.2021.3282.
Benzo[a]pyrene (BaP) is a widespread pollutant that can act as an endocrine disrupting compound (EDC) and interferes with reproductive function. The central regulatory network of the reproductive system is mediated by gonadotropin-releasing hormone (GnRH) neurons, which originate in the olfactory placode and, during ontogenesis, migrate into the hypothalamus. Given the importance of the migratory process for GnRH neuron maturation, we investigated the effect of BaP (10 µM for 24 h) on GnRH neuroblasts isolated from the human fetal olfactory epithelium (FNCB4). BaP exposure significantly reduced the mRNA level of genes implicated in FNCB4 cell migration and affected their migratory ability. Our findings demonstrate that BaP may interfere with the central neuronal network controlling human reproduction affecting GnRH neuron maturation.
苯并[a]芘（BaP）是一种广泛存在的污染物，可作为一种内分泌干扰化合物（EDC），干扰生殖功能。生殖系统的中枢调节网络由促性腺激素释放激素（GnRH）神经元介导，这些神经元起源于嗅基板，并在个体发生过程中迁移到下丘脑。鉴于迁移过程对 GnRH 神经元成熟的重要性，我们研究了 BaP（10µM，24 小时）对源自人胎儿嗅上皮的 GnRH 神经母细胞瘤（FNCB4）的影响。BaP 暴露显著降低了与 FNCB4 细胞迁移相关的基因的 mRNA 水平，并影响了它们的迁移能力。我们的研究结果表明，BaP 可能干扰控制人类生殖的中枢神经元网络，影响 GnRH 神经元的成熟。
