State Key Laboratory of Experimental Hematology, National Clinical Research Center for Blood Diseases, Institute of Hematology and Blood Diseases Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Tianjin, China.
Department of Pathology, Tianjin Huanhu Hospital, Tianjin Medical University, Tianjin, China.
Ultrastruct Pathol. 2021 Jul-Sep;45(4-5):319-334. doi: 10.1080/01913123.2021.1968090. Epub 2021 Aug 30.
To clarify the characteristics and origin of the cellular components in atherosclerosis, carotid atherosclerotic plaques (CAPs) of four patients were studied by light microscopy using hematoxylin-eosin, Congo red and alpha-smooth-muscle actin stains, and by transmission electron microscopy of different regions of CAPs. By light microscopy, CAPs were composed of 1) a fibrous cap; 2) an atherosclerotic core presenting focal fibrosis, neovascularization, hemorrhage, necrosis, chondrification and ossification; and 3) a basal band composed of a hyperplasic pseudo-media and affected tunica media. Ultrastructurally, the CAPs contained a diversity of cells including fibroblasts, myofibroblasts, osteochondrocytes, vascular smooth-muscle cells, foam cells and other myoid cells characterized by varied features of the above mentioned cells. The results indicated that CAPs were derived from a proliferation of multipotential mesenchymal stem cells, leading to the presence of degenerated foam cells and lipid-laden cells.
为了阐明动脉粥样硬化中细胞成分的特征和来源，对 4 名患者的颈动脉粥样硬化斑块（CAP）进行了研究，采用苏木精-伊红、刚果红和α-平滑肌肌动蛋白染色，以及对 CAP 不同区域进行透射电镜检查。通过光镜，CAP 由 1）纤维帽；2）呈现局灶性纤维化、新生血管形成、出血、坏死、软骨化和骨化的粥样硬化核心；和 3）由增生的假中膜和受影响的中膜组成的基底带。超微结构上，CAP 包含多种细胞，包括成纤维细胞、肌成纤维细胞、骨软骨细胞、血管平滑肌细胞、泡沫细胞和其他具有上述细胞不同特征的肌样细胞。结果表明，CAP 来源于多能间充质干细胞的增殖，导致退变的泡沫细胞和富含脂质的细胞的存在。
