State Key Laboratory of Developmental Biology of Freshwater Fish, College of Life Science, Hunan Normal University, Changsha 410081, PR China.
State Key Laboratory of Developmental Biology of Freshwater Fish, College of Life Science, Hunan Normal University, Changsha 410081, PR China.
Comp Biochem Physiol C Toxicol Pharmacol. 2021 Dec;250:109174. doi: 10.1016/j.cbpc.2021.109174. Epub 2021 Aug 28.
Ferritin H can participate in the regulation of fish immunity. Tissue-specific analysis revealed that the highest expressions of Ferritin H in parental species were observed in spleen, while peaked level of Ferritin H mRNA in hybrid fish was observed in liver. In addition, A. hydrophila challenge could sharply enhance their Ferritin H mRNA expression in liver, kidney and spleen. To further investigate their roles in immune regulation, their Ferritin H fusion proteins were produced in vitro. Ferritin H fusion proteins could exhibit a direct binding activity to A. hydrophila and endotoxin in a dose-dependent manner, restrict dissemination of A. hydrophila to tissues and abrogate inflammatory cascades. Moreover, treatment with Ferritin H fusion proteins could reduce A. hydrophila-induced lipid peroxidation. These results indicated that Ferritin H in hybrid fish elicited a similar immune regulation of A. hydrophila-induced inflammatory signals in comparison with those of its parents.
铁蛋白 H 可以参与鱼类免疫的调节。组织特异性分析显示，亲代鱼类中铁蛋白 H 的最高表达水平出现在脾脏中，而杂交鱼中铁蛋白 H mRNA 的峰值水平出现在肝脏中。此外，嗜水气单胞菌的挑战可以显著增强其肝脏、肾脏和脾脏中铁蛋白 H mRNA 的表达。为了进一步研究它们在免疫调节中的作用，我们在体外制备了它们的铁蛋白 H 融合蛋白。铁蛋白 H 融合蛋白可以以剂量依赖的方式直接与嗜水气单胞菌和内毒素结合，限制嗜水气单胞菌向组织的扩散并阻断炎症级联反应。此外，用铁蛋白 H 融合蛋白处理可以减少嗜水气单胞菌诱导的脂质过氧化。这些结果表明，与亲鱼相比，杂交鱼中的铁蛋白 H 引发了类似的免疫调节，抑制了嗜水气单胞菌诱导的炎症信号。
