State Key Laboratory of Developmental Biology of Freshwater Fish, College of Life Science, Hunan Normal University, Changsha, 410081, PR China.
Foreign Studies College, Hunan Normal University, Changsha, 410081, PR China.
Fish Shellfish Immunol. 2022 Jan;120:620-632. doi: 10.1016/j.fsi.2021.12.040. Epub 2021 Dec 27.
FerL, a multifunctional iron-storage polypeptide, not only exhibited a regulatory role in iron metabolism, but also participated in the regulation of fish immunity. In this study, ORF sequence of WR-FerL was 522 bp, encoding 173 amino acid residues. Tissue-specific analysis revealed that the highest expression of WR-FerL was detected in spleen. A. hydrophila challenge and LPS stimulation could sharply enhance WR-FerL mRNA expression in tissues and fish cells, respectively. Purified WR-FerL fusion peptide exhibited in vitro binding activity to A. hydrophila and endotoxin, limited bacterial dissemination to tissues as well as attenuated A. hydrophila-induced production of pro-inflammatory cytokines. Moreover, WR-FerL overexpression could abrogate NF-κB and TNFα promoter activity in fish cells. These results indicated that WR-FerL could play an important role in host defense against A. hydrophila infection.
铁调节蛋白 FerL 不仅在铁代谢中发挥调节作用，还参与鱼类免疫的调控。本研究中，WR-FerL 的 ORF 序列为 522bp，编码 173 个氨基酸残基。组织特异性分析显示，WR-FerL 在脾脏中的表达最高。嗜水气单胞菌攻毒和 LPS 刺激分别可显著增强组织和鱼类细胞中 WR-FerL mRNA 的表达。纯化的 WR-FerL 融合肽表现出与嗜水气单胞菌和内毒素的体外结合活性，限制了细菌向组织的扩散，并减弱了嗜水气单胞菌诱导的促炎细胞因子的产生。此外，WR-FerL 的过表达可使鱼类细胞中 NF-κB 和 TNFα 启动子活性失活。这些结果表明，WR-FerL 在宿主抵抗嗜水气单胞菌感染的防御中可能发挥重要作用。
