Kuroishikawa Takayuki, Shinmyo Daisuke, Yoshihara Akihide, Takata Goro, Watanabe Akira, Ashiuchi Makoto, Izumori Ken, Asada Yasuhiko
The United Graduate School of Agricultural Sciences, Ehime University, Tarumi, Ehime, 790-8566, Japan.
Faculty of Agriculture, Kagawa University, Miki, Kagawa, 761-0795, Japan.
Biochem Biophys Res Commun. 2021 Oct 20;575:85-89. doi: 10.1016/j.bbrc.2021.08.061. Epub 2021 Aug 24.
Some rare sugars can be potently medicinal, such as l-gulose. In this study, we present a novel alditol oxidase (fAldOx) from the soil fungus Penicillium sp. KU-1, and its application for the effective production of l-gulose. To the best of our knowledge, this is the first report of a successful direct conversion of d-sorbitol to l-gulose. We further purified it to homogeneity with a ∼108-fold purification and an overall yield of 3.26%, and also determined the effectors of fAldOx. The enzyme possessed broad substrate specificity for alditols such as erythritol (k/K, 355 m s), thus implying that the effective production of multiple rare sugars for medicinal applications may be possible.
一些稀有糖具有强大的药用价值，例如L-古洛糖。在本研究中，我们展示了一种来自土壤真菌青霉菌KU-1的新型糖醇氧化酶（fAldOx），及其在有效生产L-古洛糖中的应用。据我们所知，这是首次成功将D-山梨醇直接转化为L-古洛糖的报道。我们进一步将其纯化至同质，纯化倍数约为108倍，总产率为3.26%，并确定了fAldOx的效应物。该酶对赤藓醇等糖醇具有广泛的底物特异性（k/K，355 m s），因此意味着有可能有效生产多种用于药用的稀有糖。
