另一种 D. melanogaster 7SK snRNP。
An alternative D. melanogaster 7SK snRNP.
机构信息
Université Paris-Saclay, INSERM, CNRS, Interactions cellulaires et physiopathologie hépatique, Bât.440, 91405, Orsay, France.
MNHN, UMR CNRS 7221, 75231, Paris, France.
出版信息
BMC Mol Cell Biol. 2021 Aug 31;22(1):43. doi: 10.1186/s12860-021-00381-7.
BACKGROUND
The 7SK small nuclear RNA (snRNA) found in most metazoans is a key regulator of P-TEFb which in turn regulates RNA polymerase II elongation. Although its primary sequence varies in protostomes, its secondary structure and function are conserved across evolutionary distant taxa.
RESULTS
Here, we describe a novel ncRNA sharing many features characteristic of 7SK RNAs, in D. melanogaster. We examined the structure of the corresponding gene and determined the expression profiles of the encoded RNA, called snRNA:7SK:94F, during development. It is probably produced from the transcription of a lncRNA which is processed into a mature snRNA. We also addressed its biological function and we show that, like dm7SK, this alternative 7SK interacts in vivo with the different partners of the P-TEFb complex, i.e. HEXIM, LARP7 and Cyclin T. This novel RNA is widely expressed across tissues.
CONCLUSION
We propose that two distinct 7SK genes might contribute to the formation of the 7SK snRNP complex in D. melanogaster.
背景
在大多数后生动物中发现的 7SK 小核 RNA（snRNA）是 P-TEFb 的关键调节剂，而 P-TEFb 又反过来调节 RNA 聚合酶 II 的延伸。尽管其原始序列在原口动物中有所不同，但它的二级结构和功能在进化上相距甚远的类群中是保守的。
结果
在这里，我们在果蝇中描述了一种具有许多与 7SK RNA 特征相似的新型 ncRNA。我们研究了相应基因的结构，并确定了编码 RNA（称为 snRNA:7SK:94F）在发育过程中的表达谱。它可能是由长非编码 RNA 的转录产生的，然后被加工成成熟的 snRNA。我们还研究了它的生物学功能，并表明与 dm7SK 一样，这种替代的 7SK 在体内与 P-TEFb 复合物的不同伴侣相互作用，即 HEXIM、LARP7 和 Cyclin T。这种新型 RNA 在各种组织中广泛表达。
结论
我们提出，两个不同的 7SK 基因可能有助于果蝇中 7SK snRNP 复合物的形成。
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