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Photochemiluminescent detection of antiradical activity. I. Assay of superoxide dismutase.

作者信息

Popov I N, Lewin G, von Baehr R

机构信息

Institute of Medical Immunology, Humboldt University, Berlin, GDR.

出版信息

Biomed Biochim Acta. 1987;46(11):775-9.

PMID:3446204
Abstract

The luminol sensitized photogeneration of superoxide radicals was combined with their luminol-dependent chemiluminescent detection. The inhibition of chemiluminescence by superoxide dismutase (SOD) versus enzyme concentration in reciprocals was strongly linear. The amount of SOD notable for 50% inhibition was between 115 and 500 ng for different enzyme preparations.

摘要

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