Henley R, Worwood M
Department of Medical Biochemistry, University Hospital of Wales, Heath Park, Cardiff, UK.
J Biolumin Chemilumin. 1994 Jul-Aug;9(4):245-50. doi: 10.1002/bio.1170090402.
The generation of radicals from luminol and H2O2, in the presence of iron and iron chelates was monitored by measuring the chemiluminescence produced by further oxidation of these radicals. 2,2'-Dipyridyl enhanced the production of chemiluminescence in the presence of FeSO4, ferritin and haemosiderin but not FeCl3 or horseradish peroxidase. Nitrilotriacetic acid (NTA) enhanced chemiluminescence in the presence of both FeSO4 and FeCl3 but not ferritin or haemosiderin. The enhancement of chemiluminescence by iron chelation may have analytical applications and the process by which these iron chelates are able to generate radicals from the nitrogenous base luminol may be similar to that responsible for their toxic effects on DNA.
通过测量这些自由基进一步氧化产生的化学发光，监测在铁和铁螯合物存在下鲁米诺和过氧化氢产生自由基的情况。在硫酸亚铁、铁蛋白和血铁黄素存在下，2,2'-联吡啶增强了化学发光的产生，但在氯化铁或辣根过氧化物酶存在下则不然。氮三乙酸（NTA）在硫酸亚铁和氯化铁两者存在下增强了化学发光，但在铁蛋白或血铁黄素存在下则不然。通过铁螯合增强化学发光可能具有分析应用，并且这些铁螯合物能够从含氮碱鲁米诺产生自由基的过程可能类似于其对DNA产生毒性作用的过程。
