College of Marine Life Sciences, Department of Marine Ecology, Ocean University of China, Qingdao, 266003, China.
Laboratory for Marine Ecology and Environmental Science, Qingdao National Laboratory for Marine Science and Technology, Qingdao, 266071, China.
Environ Sci Pollut Res Int. 2022 Jan;29(5):6744-6754. doi: 10.1007/s11356-021-15950-8. Epub 2021 Aug 30.
To use microalgae for the biosequestration of carbon dioxide (CO) emitted from the coal-fired power plants, the screening of high CO tolerant microalgae and their accumulation of toxic agents have attracted significant research attention. This study evaluated 10 Chlorella strains for high CO tolerance using combined growth rates and growth periods subjected to logistic parameters. We selected LAMB 31 with high r (0.89 ± 0.10 day), high k (6.51 ± 0.19), and medium Tp (5.17 ± 0.15 day) as a candidate for CO biosequestration. Correspondingly, six genes involving carbon fixation and metabolism processes were upregulated in LAMB 31 under high CO conditions, verifying its high CO tolerant ability. LAMB 31 cultures exposed to exhaust gas of power plant under different flow rates grew well, but the high flow rate (0.6 L/h) showed inhibition effects compared with low flow rates (0.2 and 0.3 L/h) at the end of the culturing period. The toxic agents in the exhaust gas including sulfur, arsenic, and mercury accumulated in LAMB 31 biomass but were deemed safe for use in the production of both human food and animal feed based on the National Food Safety Standard in China. This study showed a complete process involving high CO tolerant microalgae screening, high CO tolerant verification, and in situ application in a power plant. Data results provide valuable information as the basis for future research studies in microalgae application on CO mitigation at emission sources.
利用微藻从燃煤电厂吸收二氧化碳（CO），筛选高 CO 耐受微藻及其积累有毒物质引起了广泛关注。本研究采用逻辑参数的组合生长率和生长周期来评估 10 株小球藻对高 CO 的耐受性。我们选择 LAMB 31 作为 CO 生物固存的候选株，因为它具有高 r（0.89 ± 0.10 天）、高 k（6.51 ± 0.19）和中 Tp（5.17 ± 0.15 天）。相应地，在高 CO 条件下，涉及碳固定和代谢过程的六个基因在 LAMB 31 中上调，证明了其高 CO 耐受能力。暴露在不同流速的电厂废气中的 LAMB 31 培养物生长良好，但在培养期末，高流速（0.6 L/h）比低流速（0.2 和 0.3 L/h）表现出抑制作用。废气中的有毒物质包括硫、砷和汞在 LAMB 31 生物质中积累，但根据中国国家食品安全标准，它们可安全用于人类食品和动物饲料的生产。本研究展示了一个完整的过程，包括高 CO 耐受微藻的筛选、高 CO 耐受的验证以及在电厂的原位应用。数据结果为未来在排放源进行 CO 减排的微藻应用研究提供了有价值的信息。
