College of Marine Life Sciences, Department of Marine Ecology, Ocean University of China, Qingdao 266003, China.
College of Marine Life Sciences, Department of Marine Ecology, Ocean University of China, Qingdao 266003, China; Laboratory for Marine Ecology and Environmental Science, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266071, China.
Sci Total Environ. 2022 Dec 20;853:158627. doi: 10.1016/j.scitotenv.2022.158627. Epub 2022 Sep 8.
The intolerance of high CO in the exhaust gas is the "bottleneck" limiting the wide application of microalgae for CO biosequestration. Around this topic, we selected high-CO-tolerant (LAMB 33 and 31) and nontolerant (LAMB 122) Chlorella strains to study their different energy metabolisms and cytoplasmic pH regulations in response to high CO. Under 40 % CO, LAMB 33 and 31 both showed elevated ATP synthesis, accelerated ATP consumption and fast cytoplasmic pH regulation while exhibiting different acclimating strategies therein: chloroplast acclimations were reflected by high chlorophyll contents in 33 but photosystem transitions in 31; faster mitochondrial acclimations occurred in 33 than in 31; cellular organic carbon mainly flowed to monosaccharide synthesis for 33 but to monosaccharide and protein synthesis for 31; and cytoplasmic pH regulation was attributed to V-ATPase in 31 but not in 33. All the above metabolic processes gradually collapsed in 122, leading to growth inhibition. Our study identified different metabolic acclimation strategies among Chlorella strains to high CO and provided new traits for breeding microalgae for CO biosequestration.
废气中高浓度 CO 的不耐受性是限制微藻用于 CO 生物固存广泛应用的“瓶颈”。围绕这一主题，我们选择了高 CO 耐受型（LAMB 33 和 31）和非耐受型（LAMB 122）小球藻菌株，研究它们在高 CO 下不同的能量代谢和细胞质 pH 调节。在 40% CO 下，LAMB 33 和 31 均表现出较高的 ATP 合成、加速的 ATP 消耗和快速的细胞质 pH 调节，同时表现出不同的适应策略：叶绿体的适应表现为 33 中高叶绿素含量，而 31 中表现为光系统转换；33 中线粒体的适应比 31 更快；细胞有机碳主要流向 33 的单糖合成，而流向 31 的单糖和蛋白质合成；细胞质 pH 调节归因于 31 中的 V-ATPase，而不是 33 中的 V-ATPase。所有这些代谢过程在 122 中逐渐崩溃，导致生长抑制。我们的研究确定了小球藻菌株对高 CO 的不同代谢适应策略，并为 CO 生物固存的微藻培育提供了新的特性。
