肺结核患者血清抑制性共刺激分子水平与固有免疫细胞因子水平的相关性。

Serum levels of inhibitory costimulatory molecules and correlations with levels of innate immune cytokines in patients with pulmonary tuberculosis.

机构信息

Department of Immunology, School of Basic Medical Sciences, Xinjiang Medical University, Urumqi, Xinjiang, China.

Postdoctoral Workstation of Traditional Chinese Medicine Hospital of Xinjiang Uygur Autonomous Region, Urumqi, Xinjiang, China.

出版信息

J Int Med Res. 2021 Aug;49(8):3000605211036832. doi: 10.1177/03000605211036832.

DOI:10.1177/03000605211036832

PMID:34463584

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8414942/

Abstract

OBJECTIVE

To analyze serum levels of inhibitory costimulatory molecules and their correlations with innate immune cytokine levels in patients with pulmonary tuberculosis (PTB).

METHODS

Data for 280 PTB patients and 280 healthy individuals were collected. Serum levels of immune molecules were measured using ELISA. Univariate, multivariate, subgroup, matrix correlation, and receiver operating characteristic curve analyses were performed.

RESULTS

Host, environment, lifestyle, clinical features, and medical history all influenced PTB. Serum levels of soluble programmed death ligand 1 (sPD-L1), soluble T-cell immunoglobulin- and mucin-domain-containing molecule 3 (sTim-3), soluble galectin-9 (sGal-9), interleukin (IL)-4, and IL-33 were significantly higher in patients with PTB, while levels of IL-12, IL-23, IL-18, and interferon (IFN)-γ were significantly lower. Serum levels of sTim-3 were higher in alcohol users. Levels of sTim-3 were negatively correlated with those of IL-12. Levels of IL-12, IL-23, and IL-18 were positively correlated with those of IFN-γ, while levels of IL-12 were negatively correlated with those of IL-4. The areas under the curve of sPD-L1, sTim-3, sGal-9, IL-12, IL-23, IL-18, IFN-γ, IL-4, and IL-33 for identifying PTB were all >0.77.

CONCLUSIONS

Inhibitory costimulatory molecules may be targets for controlling PTB. Immune molecules may be helpful for diagnosis of PTB.

摘要

目的

分析肺结核（PTB）患者血清抑制性共刺激分子水平及其与固有免疫细胞因子水平的相关性。

方法

收集 280 例 PTB 患者和 280 例健康对照者的数据。采用 ELISA 法检测免疫分子的血清水平。进行单变量、多变量、亚组、矩阵相关性和受试者工作特征曲线分析。

结果

宿主、环境、生活方式、临床特征和病史均影响 PTB。PTB 患者可溶性程序性死亡配体 1（sPD-L1）、可溶性 T 细胞免疫球蛋白和粘蛋白结构域 3（sTim-3）、可溶性半乳糖凝集素 9（sGal-9）、白细胞介素（IL）-4 和 IL-33 血清水平显著升高，而 IL-12、IL-23、IL-18 和干扰素（IFN）-γ 血清水平显著降低。酒精使用者 sTim-3 血清水平较高。sTim-3 水平与 IL-12 水平呈负相关。IL-12、IL-23 和 IL-18 水平与 IFN-γ 水平呈正相关，而 IL-12 水平与 IL-4 水平呈负相关。sPD-L1、sTim-3、sGal-9、IL-12、IL-23、IL-18、IFN-γ、IL-4 和 IL-33 鉴别 PTB 的曲线下面积均>0.77。