Core Facility of Instrument, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, School of Basic Medicine, Peking Union Medical College, Beijing, China.
Department of Obstetrics and Gynecology, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences, Beijing, China.
Proteomics. 2021 Oct;21(20):e2000321. doi: 10.1002/pmic.202000321. Epub 2021 Sep 12.
Gingival crevicular fluid (GCF) is an integral part of oral fluid that plays a special role in maintaining the structure of junctional epithelium and defending against bacterial infection. In this study, we comprehensively analysed the composition of the human GCF proteome and metaproteome simultaneously to obtain multidimensional information about GCF. A total of 3680 human proteins (2540 with at least two unique peptides) were identified in the normal GCF sample, and their functions were mainly associated with immune function and inflammation. Among these proteins, 1874 proteins could be quantified by the iBAQ algorithm, and their abundances spanned a dynamic range of six orders of magnitude. For the GCF metaproteome, a total of 3082 proteins and 69 genera were found. In addition, 16 genera were not identified by GCF metagenomic analysis. Compared to the saliva metaproteome, 32 genera were found to be in common. The protein quantitative analysis showed that the abundance of GCF metaproteome contributed to approximately 4.17% of the total GCF proteome. The top three most abundant genera were Fusobacterium, Corynebacterium, and Leptotrichia. The above data will be useful for future research on GCF-related diseases.
牙龈沟液（GCF）是口腔液的一个组成部分，在维持结合上皮结构和抵御细菌感染方面起着特殊作用。在这项研究中，我们全面分析了人类 GCF 蛋白质组和宏蛋白质组的组成，以获得有关 GCF 的多维信息。在正常 GCF 样本中鉴定出了 3680 个人类蛋白质（至少有两个独特肽的 2540 个），其功能主要与免疫功能和炎症有关。在这些蛋白质中，1874 种蛋白质可以通过 iBAQ 算法进行定量，其丰度跨越了六个数量级的动态范围。对于 GCF 宏蛋白质组，共发现了 3082 种蛋白质和 69 属。此外，有 16 个属未通过 GCF 宏基因组分析鉴定。与唾液宏蛋白质组相比，发现有 32 个属是共同的。蛋白质定量分析表明，GCF 宏蛋白质组的丰度约占 GCF 蛋白质组总量的 4.17%。最丰富的三个属是梭杆菌属、棒状杆菌属和勒克氏菌属。上述数据将有助于未来研究与 GCF 相关的疾病。
