Department of Periodontology and Oral Biology, Henry M. Goldman School of Dental Medicine, Boston University Medical Center, Boston, MA 02118-2648, USA.
J Periodontal Res. 2012 Apr;47(2):248-62. doi: 10.1111/j.1600-0765.2011.01429.x. Epub 2011 Oct 26.
Gingival crevicular fluid has been of major interest for many decades as a valuable body fluid that may serve as a source of biomarkers for both periodontal and systemic diseases. Owing to its very small sample size, submicroliter volumes, identification of its protein composition by classical biochemical methods has been limited. The advent of highly sensitive mass spectrometric technology has permitted large-scale identification of protein components of many biological samples. This technology has been employed to identify the protein composition of gingival crevicular fluid from inflamed and periodontal sites. In this report, we present a proteome data set of gingival crevicular fluid from healthy periodontium sites.
A combination of a periopaper collection method with application of multidimensional protein separation and mass spectrometric technology led to a large-scale documentation of the proteome of gingival crevicular fluid from healthy periodontium sites.
The approaches used have culminated in identification of 199 proteins in gingival crevicular fluid of periodontally healthy sites. The present gingival crevicular fluid proteome from healthy sites was compared and contrasted with those proteomes of gingival crevicular fluid from inflamed and periodontal sites, as well as serum. The cross-correlation of the gingival crevicular fluid and plasma proteomes permitted dissociation of the 199 identified gingival crevicular fluid proteins into 105 proteins (57%) that can be identified in plasma and 94 proteins (43%) that are distinct and unique to the gingival crevicular fluid microenvironment. Such analysis also revealed distinctions in protein functional categories between serum proteins and those specific to the gingival crevicular fluid microenvironment.
Firstly, the data presented herein provide the proteome of gingival crevicular fluid from periodontally healthy sites through establishment of innovative analytical approaches for effective analysis of gingival crevicular fluid from periopapers both at the level of complete elusion and with removal of abundant albumin, which restricts identification of low-abundant proteins. Secondly, it adds significantly to the knowledge of gingival crevicular fluid composition and highlights new groups of proteins specific to the gingival crevicular fluid microenvironment.
几十年来，牙龈沟液一直是人们关注的焦点，因为它是一种有价值的体液，可以作为牙周病和系统性疾病生物标志物的来源。由于其样本量非常小，体积在亚微升范围内，因此传统的生化方法对其蛋白质组成的鉴定受到限制。高灵敏度质谱技术的出现使得对许多生物样本的蛋白质成分进行大规模鉴定成为可能。该技术已被用于鉴定来自炎症和牙周部位的牙龈沟液的蛋白质组成。在本报告中，我们展示了来自健康牙周部位的牙龈沟液的蛋白质组数据集。
一种牙周纸收集方法与多维蛋白质分离和质谱技术的结合，导致了对来自健康牙周部位的牙龈沟液的蛋白质组进行了大规模的记录。
所采用的方法最终确定了 199 种蛋白质在牙周健康部位的牙龈沟液中。本研究还比较和对比了来自健康部位、炎症部位和血清的牙龈沟液蛋白质组。牙龈沟液和血浆蛋白质组的交叉相关性允许将 199 种鉴定出的牙龈沟液蛋白质分为 105 种（57%）可以在血浆中鉴定出的蛋白质和 94 种（43%）在牙龈沟液微环境中独特的蛋白质。这种分析还揭示了血清蛋白和牙龈沟液微环境中特定蛋白之间在蛋白质功能类别上的差异。
首先，本文提供了通过建立创新的分析方法来分析牙周纸中的牙龈沟液，从而确定牙周健康部位的牙龈沟液蛋白质组，该方法可以有效地分析完整洗脱和去除丰富白蛋白的牙龈沟液，这限制了低丰度蛋白质的鉴定。其次，它大大增加了对牙龈沟液组成的认识，并突出了特定于牙龈沟液微环境的新蛋白质组。
