Dybdahl H, Ledet T
University Institute of Pathology, Aarhus Kommunehospital, Denmark.
Diabetologia. 1987 Nov;30(11):882-6.
A morphometric study was performed on histological sections of the extramural coronary arteries from 10 Type 2 (non-insulin-dependent) diabetic patients and 10 non-diabetic subjects, matched for age and sex. Standardised samples were taken from the two coronary arteries and the fractional contents of Periodic Acid-Schiff-positive material, acid mucopolysaccharides and connective tissue was determined in the arterial tunica media from the two groups using a point-counting technique. The thickness of tunica intima and tunica media was obtained by micrometric measurements. There was no difference in the thickness of tunica intima with and without atherosclerotic plaque between the vessels from diabetic and nondiabetic hearts. However, the thickness of tunica media was significantly reduced in arteries from the diabetic patients (2p less than 0.01). In tunica media of the diabetic patients there was a significant increase (50%) in the amount of Periodic Acid-Schiff-positive material (2p less than 0.01), whereas the content of acid mucopolysaccharides (alcian-blue positive) was significantly reduced (2p less than 0.02). These changes in tunica media were similar in areas below and outside the intimal atherosclerotic plaques. The content of connective tissue was found to be significantly increased in the arteries from the diabetic patients, but only in areas outside atherosclerotic plaques (2p less than 0.025). There was no correlation between the observed changes in tunica media and the known duration of diabetes. The results obtained in the present study may be ascribed to the presence of a non-atherosclerotic diabetic macroangiopathy.
对10名2型（非胰岛素依赖型）糖尿病患者和10名年龄及性别相匹配的非糖尿病受试者的壁外冠状动脉组织切片进行了形态计量学研究。从两根冠状动脉获取标准化样本，采用点计数技术测定两组动脉中膜中过碘酸希夫阳性物质、酸性粘多糖和结缔组织的含量分数。通过测微测量获得内膜和中膜的厚度。糖尿病心脏和非糖尿病心脏血管中，有和没有动脉粥样硬化斑块时的内膜厚度没有差异。然而，糖尿病患者动脉的中膜厚度显著降低（P<0.01）。糖尿病患者中膜中过碘酸希夫阳性物质的量显著增加（50%）（P<0.01），而酸性粘多糖（阿尔辛蓝阳性）的含量显著降低（P<0.02）。中膜的这些变化在内膜动脉粥样硬化斑块下方和外部区域相似。发现糖尿病患者动脉中的结缔组织含量显著增加，但仅在动脉粥样硬化斑块外部区域（P<0.025）。中膜观察到的变化与已知糖尿病病程之间没有相关性。本研究获得的结果可能归因于非动脉粥样硬化性糖尿病大血管病变的存在。
