Nooranizadeh Mohammad Hossein, Rahmanifar Farhad, Ahmadloo Somayeh, Shaaban Zahra, Jafarzadeh Shirazi Mohammad Reza, Tamadon Amin
Stem Cells Technology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.
Department of Basic Sciences, School of Veterinary Medicine, Shiraz University, Shiraz, Iran.
Galen Med J. 2018 May 29;7:e1070. doi: 10.22086/gmj.v0i0.1070. eCollection 2018.
Hypothalamic () and kiss1/kisspeptin systems play roles in reproductive processes. This study was conducted to evaluate changes in and genes expression in the arcuate nucleus (ARC) of the hypothalamus and its relationship with polycystic ovary syndrome (PCOS) in rats.
In the current experimental study, 24 female rats were randomly and equally allocated into nulliparous and primiparous groups and then were divided into two subgroups of PCOS and control. PCOS was induced by exposure to continuous light. Sex-related hormones were evaluated by radioimmunoassay or immunoradiometric assay. Expressions of and gene in the ARC of the hypothalamus of the rats were evaluated by real-time PCR. Histomorphometric alterations of ovaries were compared between groups.
Number of tertiary follicles and their size and number of atretic follicles in the PCOS subgroups were more than those in the controls (P<0.05) whereas the number of secondary follicles and corpus luteum in the PCOS subgroups were lower than those in the controls (P<0.05). Antrum and total diameters of tertiary follicles in the PCOS subgroups were greater and granulosa layer diameter was lower than those in the controls (P<0.05). The mRNA expression in the PCOS subgroups was 6.5-fold in nulliparous and 3.5-fold in primiparous groups more than their controls' pairs (P<0.05). However, parity did not affect the expression of gene (P>0.05). The mRNA expression in the PCOS and control subgroups was not significantly different (P>0.05).
Overexpression of gene after PCOS induction in the ARC of the hypothalamus may link to metabolic disorders of induced PCOS in the rats. However, alteration in the mRNA expression after PCOS induction was not observed in the rats.
下丘脑促性腺激素释放激素（GnRH）和Kiss1/ Kisspeptin系统在生殖过程中发挥作用。本研究旨在评估大鼠下丘脑弓状核（ARC）中GnRH和Kiss1基因表达的变化及其与多囊卵巢综合征（PCOS）的关系。
在当前的实验研究中，24只雌性大鼠被随机且平均分为未生育组和经产组，然后再分为PCOS和对照组两个亚组。通过持续光照诱导PCOS。采用放射免疫分析或免疫放射分析评估性激素水平。通过实时PCR评估大鼠下丘脑ARC中GnRH和Kiss1基因的表达。比较各组卵巢的组织形态计量学改变。
PCOS亚组中三级卵泡的数量、大小以及闭锁卵泡的数量均多于对照组（P<0.05），而PCOS亚组中二级卵泡和黄体的数量低于对照组（P<0.05）。PCOS亚组中三级卵泡的卵泡腔和总直径更大，颗粒层直径低于对照组（P<0.05）。PCOS亚组中GnRH mRNA表达在未生育组比其对照组高6.5倍，在经产组高3.5倍（P<0.05）。然而，产次不影响Kiss1基因的表达（P>0.05）。PCOS和对照组亚组中Kiss1 mRNA表达无显著差异（P>0.05）。
PCOS诱导后下丘脑ARC中GnRH基因的过表达可能与大鼠诱导性PCOS的代谢紊乱有关。然而，在大鼠中未观察到PCOS诱导后Kiss1 mRNA表达的改变。
