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产仔数增加和哺乳强度抑制大鼠弓状核中 KiSS-1 mRNA 的表达。

Increased litter size and suckling intensity inhibit KiSS-1 mRNA expression in rat arcuate nucleus.

机构信息

Department of Animal Sciences, College of Agriculture, Shiraz University, Shiraz, Iran.

Infertility Research Center, Shiraz University of Medical Sciences, Shiraz, Iran ; Transgenic Technology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.

出版信息

Iran J Basic Med Sci. 2014 Aug;17(8):600-5.

PMID:25422754
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4240795/
Abstract

OBJECTIVES

The effect of litter size and suckling intensity on the expression of KiSS-1 mRNA in the arcuate nucleus (ARC) of rats were evaluated.

MATERIALS AND METHODS

Thirty two pregnant and four non-lactating ovariectomized (as control group) rats were used in this experiment. Lactating rats were allotted to eight equal groups. In three groups, litter size was adjusted to 5, 10, or 15 pups upon parturition and allowed to suckle their pups continuously by 8 days postpartum. In the other three groups, litter size was adjusted to five upon birth; the pups were separated from the dams for 6 hr on day 8 postpartum, after which the pups were allowed to suckle their dams for 2.5, 5, or 7.5 min prior to killing the dams. Two groups of lactating rats with either 10 or 15 pups were separated from their pups for 6 hr on day 8 postpartum, after which the pups were allowed to suckle their dams for 5 min before the dams were killed on day 8 postpartum. The ARC was removed and the expression of KiSS-1 mRNA was evaluated, using real-time PCR.

RESULTS

The expression of KiSS-1 mRNA in the ARC was decreased as the litter size and intensity of suckling stimulus were increased. The effect of suckling intensity on the expression of KiSS-1 mRNA was more pronounced than that of litter size.

CONCLUSION

Increased litter size and suckling intensity decreased KiSS-1 mRNA expression in the ARC which may contribute to lactation anestrus in rat.

摘要

目的

评估胎鼠数量和哺乳强度对大鼠弓状核（ARC）中 Kiss-1 mRNA 表达的影响。

材料与方法

本实验使用了 32 只怀孕和 4 只未哺乳的去卵巢（作为对照组）大鼠。哺乳期大鼠被分为 8 个相等的组。在 3 个组中，胎鼠数量在分娩时调整为 5、10 或 15 只，并允许其在产后 8 天内连续哺乳；在另外 3 个组中，胎鼠数量在出生时调整为 5 只；在产后第 8 天，将幼鼠与母鼠分离 6 小时，然后让幼鼠分别吸吮母鼠 2.5、5 或 7.5 分钟，然后处死母鼠。两组哺乳期大鼠，每组 10 或 15 只，在产后第 8 天与幼鼠分离 6 小时，然后让幼鼠吸吮母鼠 5 分钟，然后在产后第 8 天处死母鼠。取出 ARC，使用实时 PCR 评估 Kiss-1 mRNA 的表达。

结果

随着胎鼠数量和哺乳刺激强度的增加，ARC 中 Kiss-1 mRNA 的表达减少。哺乳强度对 Kiss-1 mRNA 表达的影响比胎鼠数量更为显著。

结论

胎鼠数量增加和哺乳强度增加会降低 ARC 中 Kiss-1 mRNA 的表达，这可能导致大鼠泌乳静止。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/531c/4240795/509e773baf9b/IJBMS-17-600_F3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/531c/4240795/b468e5131403/IJBMS-17-600_F1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/531c/4240795/6aad0882d852/IJBMS-17-600_F2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/531c/4240795/509e773baf9b/IJBMS-17-600_F3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/531c/4240795/b468e5131403/IJBMS-17-600_F1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/531c/4240795/6aad0882d852/IJBMS-17-600_F2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/531c/4240795/509e773baf9b/IJBMS-17-600_F3.jpg

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Neurosci Res. 2012 Sep;74(1):10-6. doi: 10.1016/j.neures.2012.05.011. Epub 2012 Jun 9.
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Iran J Basic Med Sci. 2018 Aug;21(8):848-855. doi: 10.22038/IJBMS.2018.28489.6905.
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