Sefidgar Tehrani Maryam, Amirian Malihe, Jalali Mohsen, Attaranzadeh Armin, Fazel Alireza, Ebrahimzadeh-Bideskan Alireza
Department of Anatomy and Cell Biology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
Department of Reproductive Medicine and Gynecology, Milad Infertility Center, Mashhad, Iran.
Galen Med J. 2018 Jun 12;7:e1107. doi: 10.22086/gmj.v0i0.1107. eCollection 2018.
New methods are needed to optimize intracytoplasmic sperm injection (ICSI) outcomes in oligoasthenozoospermic (OAS) men. We evaluated the level of DNA fragment index (DFI) in OAS men and its impact on ICSI outcomes. In addition, we used the zeta potential method for sperm selection to investigate the efficacy of this technique in improving ICSI outcomes.
This cross-sectional study was performed on 95 couples. Sperm parameters and sperm DNA fragmentation (SDF) were measured. The couples were divided into the following 3 groups: group I (n=30) where SDF was between 15% and 30%, and routine sperm was selected on the basis of motility and morphology; group II (n=34) where SDF was more than 30%, and the routine sperm selection method was applied on the basis of motility and morphology; and group III (n=31) where SDF was more than 30%, and the sperm selection was performed on the basis of the zeta method. The fertilization rate, embryo development, embryo quality, and implantation rate were evaluated in these 3 groups.
The fertilization rate was significantly higher in group I compared with group II (P<0.05). The embryo development rate in group I was significantly higher than that in group II (P<0.001) and group III (P<0.05), and it was significantly lower in group II compared with group III (P<0.05). The embryo quality was higher in group III compared with group II (P<0.01). The implantation rate in group I was significantly higher than that in group II (P<0.05) and group III (P<0.05).
The present study indicated that a higher level of SDF has an adverse effect on the ICSI outcome. Furthermore, the zeta potential technique can be a useful method for sperm selection in OAS men.
需要新方法来优化少弱精子症（OAS）男性的胞浆内单精子注射（ICSI）结果。我们评估了OAS男性的DNA片段指数（DFI）水平及其对ICSI结果的影响。此外，我们使用zeta电位法进行精子筛选，以研究该技术在改善ICSI结果方面的效果。
对95对夫妇进行了这项横断面研究。测量了精子参数和精子DNA碎片率（SDF）。这些夫妇被分为以下3组：第一组（n = 30），SDF在15%至30%之间，根据活力和形态选择常规精子；第二组（n = 34），SDF超过30%，根据活力和形态应用常规精子筛选方法；第三组（n = 31），SDF超过30%，根据zeta方法进行精子筛选。评估了这3组的受精率、胚胎发育、胚胎质量和着床率。
第一组的受精率显著高于第二组（P < 0.05）。第一组的胚胎发育率显著高于第二组（P < 0.001）和第三组（P < 0.05），第二组显著低于第三组（P < 0.05）。第三组的胚胎质量高于第二组（P < 0.01）。第一组的着床率显著高于第二组（P < 0.05）和第三组（P < 0.05）。
本研究表明，较高水平的SDF对ICSI结果有不利影响。此外，zeta电位技术可能是OAS男性精子筛选的一种有用方法。
