Jeddi Farhad, Alipour Shahriar, Najafzadeh Nowruz, Dadashpour Mehdi, Pouremamali Farhad, Sadeghi Mohammad Reza, Samadi Nasser, Soozangar Narges, Khamaneh Amir Mahdi
Research Laboratory for Embryology and Stem Cells, Department of Anatomical Sciences and Pathology, School of Medicine, Ardabil University of Medical Sciences, Ardabil, Iran.
Department of Biochemistry, Faculty of Medicine, Urmia University of Medical Sciences, Urmia, Iran.
Galen Med J. 2019 Jan 25;8:e1329. doi: 10.31661/gmj.v8i0.1329. eCollection 2019.
MicroRNAs (miRNAs) play critical roles in different pathological processes including cancer development and progression. To find novel molecular diagnostic and prognostic markers and promising therapeutic tools for gastric cancer (GC), we aimed to investigate the relationship of the expression levels of miR-28-5p or miR-200a-3p with the clinicopathological criteria and to explore their impacts on the progression of human GC.
Quantitative RT-PCR was performed to analyze miR-28 and miR-200a expression in 60 GC and 60 non-GC tissue samples.
Our results revealed that the expressions of miR-200a and miR-28 were significantly downregulated in GC in comparison with non- GC tissues. Tumors with low miR-28 expression had larger tumor size, more advanced histological grade, and a higher incidence of lymph node and distal metastasis than the tumors with high miR-28 expressions. Furthermore, receiver operating characteristic (ROC) analyses demonstrate that the expression of miR-28 is a predictive biomarker allows predicting the histological grade, tumor size, and occurrence of nodal and distal metastases. We also found a significant inverse association between miR-200a expression and the rate of lymph node metastasis (p = 0.010, r = -0.334).
Our findings suggest that the miR-28 and miR-200a have tumor-suppressor functions and may be considered as potential biomarkers for gastric cancer diagnosis and prognosis.
微小RNA（miRNA）在包括癌症发生和发展在内的不同病理过程中发挥关键作用。为了寻找胃癌（GC）新的分子诊断和预后标志物以及有前景的治疗工具，我们旨在研究miR-28-5p或miR-200a-3p表达水平与临床病理标准的关系，并探讨它们对人类GC进展的影响。
采用定量逆转录聚合酶链反应（qRT-PCR）分析60例GC组织和60例非GC组织样本中miR-28和miR-200a的表达。
我们的结果显示，与非GC组织相比，miR-200a和miR-28在GC中的表达显著下调。miR-28低表达的肿瘤比miR-28高表达的肿瘤具有更大的肿瘤大小、更高的组织学分级以及更高的淋巴结和远处转移发生率。此外，受试者工作特征（ROC）分析表明，miR-28的表达是一种预测生物标志物，可用于预测组织学分级、肿瘤大小以及淋巴结和远处转移的发生情况。我们还发现miR-200a表达与淋巴结转移率之间存在显著的负相关（p = 0.010，r = -0.334）。
我们的研究结果表明，miR-28和miR-200a具有肿瘤抑制功能，可能被视为胃癌诊断和预后的潜在生物标志物。
