Takeyama T, Suzuki I, Ohno N, Oikawa S, Sato K, Ohsawa M, Yadomae T
Tokyo College of Pharmacy, Japan.
J Pharmacobiodyn. 1987 Nov;10(11):644-51. doi: 10.1248/bpb1978.10.644.
The antitumor mechanism of grifolan NMF-5N, a beta-1,3-glucan obtained from mycelia of Grifola frondosa, was examined. Grifolan NMF-5N did not show direct cytocidal effect on cultured tumor cells. However, intraperitoneal injection of grifolan NMF-5N increased the number of peritoneal exudate cells and peritoneal adherent cells which showed cytostatic activity towards syngeneic tumor cells. In an in vivo assay, the administration of carrageenan, an inhibitor of macrophage function, reduced the antitumor activity of grifolan NMF-5N. The delayed-type hypersensitivity reaction was augmented in the grifolan NMF-5N-administered mice. The administration of NMF-5N augmented the induction of cytotoxic T cells but the antitumor activity of grifolan NMF-5N was reduced in athymic nu/nu mice. In addition, the treatment with anti-Thy 1,2 antibody and complement C' of spleen cells taken from mice which showed regression of tumor due to grifolan NMF-5N, reduced the neutralizing effect in Winn assay. These results suggested that grifolan NMF-5N shows antitumor activity via host-mediated mechanisms and both macrophages and T cells play important roles in the mechanisms.
研究了从灰树花菌丝体中获得的β-1,3-葡聚糖——灰树花多糖NMF-5N的抗肿瘤机制。灰树花多糖NMF-5N对培养的肿瘤细胞未显示出直接的杀细胞作用。然而，腹腔注射灰树花多糖NMF-5N可增加对同基因肿瘤细胞具有细胞抑制活性的腹腔渗出细胞和腹腔黏附细胞的数量。在体内试验中，巨噬细胞功能抑制剂角叉菜胶的给药降低了灰树花多糖NMF-5N的抗肿瘤活性。在给予灰树花多糖NMF-5N的小鼠中，迟发型超敏反应增强。给予NMF-5N增强了细胞毒性T细胞的诱导，但在无胸腺裸鼠中灰树花多糖NMF-5N的抗肿瘤活性降低。此外，用抗Thy 1,2抗体和补体C'处理因灰树花多糖NMF-5N而肿瘤消退的小鼠的脾细胞，在Winn试验中降低了中和作用。这些结果表明，灰树花多糖NMF-5N通过宿主介导的机制显示抗肿瘤活性，巨噬细胞和T细胞在该机制中均起重要作用。
