Department of Chemistry, University of Mumbai, Mumbai, India.
Nat Prod Res. 2023 Feb;37(3):494-497. doi: 10.1080/14786419.2021.1971978. Epub 2021 Sep 1.
This article records for the first time the isolation of Ursolic acid from the leaves of (Roxb.) Bosser (Family: Rubiaceae) using ultrasonic waves. This bioactive triterpenic acid was isolated without its isomer, oleanolic acid, in a very convenient way with good yield. The structure was identified by means of one dimensional Nuclear Magnetic Resonance (NMR) spectroscopic techniques like H NMR, C NMR, distortionless enhancement by polarization transfer (DEPT) and two dimensional NMR spectroscopic method for example, heteronuclear single quantum coherence (HSQC). It was also assayed for antidiabetic and antioxidant potencies. About 71.5 mg of pure ursolic acid was isolated from 2.6 grams of ethyl acetate soluble fraction using sono-maceration as an extraction technique.
本文首次记录了使用超声波从（Roxb.）Bosser（茜草科）叶中分离出熊果酸。这种生物活性三萜酸是在非常方便的条件下分离出来的，没有其异构体齐墩果酸，产率也很好。通过一维核磁共振（NMR）光谱技术，如 H NMR、C NMR、无畸变极化转移增强（DEPT）和二维 NMR 光谱方法，例如异核单量子相干（HSQC），确定了其结构。它还被检测到具有抗糖尿病和抗氧化作用。采用超声提取技术，从 2.6 克乙酸乙酯可溶部分中分离出约 71.5mg 的纯熊果酸。
