基于光片荧光显微镜和光相干断层扫描的多模态高分辨率胚胎成像。
Multimodal high-resolution embryonic imaging with light sheet fluorescence microscopy and optical coherence tomography.
出版信息
Opt Lett. 2021 Sep 1;46(17):4180-4183. doi: 10.1364/OL.430202.
Abstract
A high-resolution imaging system combining optical coherence tomography (OCT) and light sheet fluorescence microscopy (LSFM) was developed. LSFM confined the excitation to only the focal plane, removing the out of plane fluorescence. This enabled imaging a murine embryo with higher speed and specificity than traditional fluorescence microscopy. OCT gives information about the structure of the embryo from the same plane illuminated by LSFM. The co-planar OCT and LSFM instrument was capable of performing co-registered functional and structural imaging of mouse embryos simultaneously.
摘要
我们开发了一种结合光学相干断层扫描（OCT）和光片荧光显微镜（LSFM）的高分辨率成像系统。LSFM 将激发光限制在仅焦平面内，去除了非焦平面的荧光。这使得能够以比传统荧光显微镜更高的速度和特异性对小鼠胚胎进行成像。OCT 从 LSFM 照亮的同一平面提供有关胚胎结构的信息。共面 OCT 和 LSFM 仪器能够同时对小鼠胚胎进行功能和结构的共定位成像。
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2
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Dev Biol. 2016 Nov 15;419(2):229-236. doi: 10.1016/j.ydbio.2016.09.011. Epub 2016 Sep 23.
3
Optical coherence tomography for embryonic imaging: a review.光学相干断层扫描在胚胎成像中的应用：综述。
J Biomed Opt. 2016 May 1;21(5):50902. doi: 10.1117/1.JBO.21.5.050902.
4
Direct four-dimensional structural and functional imaging of cardiovascular dynamics in mouse embryos with 1.5 MHz optical coherence tomography.利用1.5兆赫光学相干断层扫描对小鼠胚胎心血管动力学进行直接四维结构和功能成像。
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5
The renaissance of developmental biology.发育生物学的复兴。
PLoS Biol. 2015 May 6;13(5):e1002149. doi: 10.1371/journal.pbio.1002149. eCollection 2015 May.
6
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