极亮的近红外发射自发闪烁荧光团实现活细胞中的比率多色纳米显微镜技术。
Extremely Bright, Near-IR Emitting Spontaneously Blinking Fluorophores Enable Ratiometric Multicolor Nanoscopy in Live Cells.
作者信息
Tyson Jonathan, Hu Kevin, Zheng Shuai, Kidd Phylicia, Dadina Neville, Chu Ling, Toomre Derek, Bewersdorf Joerg, Schepartz Alanna
机构信息
Department of Chemistry, University of California, Berkeley, California 94720, United States.
Department of Chemistry, Yale University, New Haven, Connecticut 06520, United States.
出版信息
ACS Cent Sci. 2021 Aug 25;7(8):1419-1426. doi: 10.1021/acscentsci.1c00670. Epub 2021 Jul 23.
New bright, photostable, emission-orthogonal fluorophores that blink without toxic additives are needed to enable multicolor, live-cell, single-molecule localization microscopy (SMLM). Here we report the design, synthesis, and biological evaluation of Yale, a photostable, near-IR-emitting fluorophore that achieves these goals in the context of an exceptional quantum yield (0.59). When used alongside HMSiR, Yale enables simultaneous, live-cell, two-color SMLM of two intracellular organelles (ER + mitochondria) with only a single laser and no chemical additives.
为了实现多色、活细胞、单分子定位显微镜（SMLM），需要新型的明亮、光稳定、发射正交且无需有毒添加剂即可实现闪烁的荧光团。在此，我们报告了Yale的设计、合成及生物学评估，Yale是一种光稳定的近红外发射荧光团，在出色的量子产率（0.59）情况下实现了这些目标。与HMSiR一起使用时，Yale仅用单一激光且无需化学添加剂，就能对两种细胞内细胞器（内质网 + 线粒体）进行同步、活细胞、双色SMLM。
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