School of Physical Science and Technology, ShanghaiTech University, Shanghai, China.
Methods Mol Biol. 2021;2347:167-176. doi: 10.1007/978-1-0716-1574-4_15.
Natural silk protein fibers have shown a great attraction to the researchers due to the extraordinary mechanical property, biocompatibility, and functional diversity. Unfortunately, the low yield and unevenness have hampered the scale use of the natural silk fibers. Herein, the appearance of the bioinspired artificial spinning strategy offers an effective way to fabricate silk fibers with controllable structures and functionality. This chapter describes an experimental method to prepare silk protein fibers on a large scale and summarizes the method to investigate the effects of the structure-property relationship of the recombinant protein fibers.
天然丝蛋白纤维由于其非凡的机械性能、生物相容性和功能多样性，引起了研究人员的极大关注。不幸的是，低产量和不均匀性阻碍了天然丝纤维的规模化应用。在此，仿生人工纺丝策略的出现为制备具有可控结构和功能的丝纤维提供了一种有效的方法。本章描述了一种大规模制备丝蛋白纤维的实验方法，并总结了研究重组蛋白纤维的结构-性能关系的方法。
